Department of Mechanical & Aerospace Engineering, The Ohio State University, Columbus, OH 43210, United States of America.
Physiol Meas. 2020 Sep 10;41(8):085005. doi: 10.1088/1361-6579/abaa55.
To probe the distribution of electrical properties in tumor-bearing human hepatic tissues with metastatic colorectal cancer.
Electrochemical impedance spectroscopy (EIS) and a non-contact electromagnetic probe were used for distinguishing spatial heterogeneities in fresh, unfixed human hepatic tissues ex vivo from patients with metastatic colorectal cancer (CRC).
Point-wise EIS measurements reported over a frequency range of 100 Hz-1 MHz showed that the interface tissue between visible tumor and normal tissue exhibits an electrically different domain (p < 0.05) from both normal tissue (over 100 Hz-100 kHz) and tumor tissue (over 100 Hz-1 MHz). Observations of the microstructure on tumor-bearing hepatic tissue from hematoxylin and eosin stained images and the equivalent circuit modelling were used to validate the impedance measurements and characterize previously unidentified interfacial domain between normal and tumor tissue. Lastly, in a proof of concept study, a new in-house designed non-contact electromagnetic probe, as opposed to the invasive EIS measurements, was demonstrated for distinguishing tumor tissue from the normal tissue in a hepatic tissue specimen from a patient with metastatic CRC.
EIS measurements, correlated with histological observations, show potential for mapping electrical properties in tumor-bearing human hepatic tissue.
探讨转移性结直肠癌患者荷瘤人肝组织的电学特性分布。
采用电化学阻抗谱（EIS）和非接触电磁探头，对转移性结直肠癌患者新鲜、未固定的人离体肝组织进行体外区分，以研究空间异质性。
在 100 Hz-1 MHz 的频率范围内进行的逐点 EIS 测量表明，肉眼可见肿瘤与正常组织之间的界面组织在电学上与正常组织（100 Hz-100 kHz 以上）和肿瘤组织（100 Hz-1 MHz 以上）不同（p < 0.05）。荷瘤肝组织的苏木精和伊红染色图像的微观结构观察和等效电路模型用于验证阻抗测量，并对正常组织和肿瘤组织之间以前未识别的界面域进行特征描述。最后，在一项概念验证研究中，与侵入性 EIS 测量相比，新设计的非接触电磁探头用于区分转移性结直肠癌患者肝组织标本中的肿瘤组织与正常组织。
与组织学观察相关的 EIS 测量显示，有可能绘制荷瘤人肝组织的电学特性图。
