Zhao Qiang, Bai Yunpeng, Liu Dan, Zhao Nan, Gao Huiyuan, Zhang Xiaozhe
KeyLaboratory of Structure-Based Drug Design & Discovery of Ministry of Education, Shenyang Pharmaceutical University, Shenyang, China.
CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
J Ginseng Res. 2020 Sep;44(5):680-689. doi: 10.1016/j.jgr.2019.05.010. Epub 2019 May 29.
Peptides have diverse and important physiological roles in plants and are ideal markers for species identification. It is unclear whether there are specific peptides in L. (PQ). The aims of this study were to identify Quinetides, a series of diverse posttranslational modified native peptides of the ribonuclease-like storage protein (ginseng major protein), from PQ to explore novel peptide markers and develop a new method to distinguish PQ from
We used different fragmentation modes in the LTQ Orbitrap analysis to identify the enriched Quinetide targets of PQ, and we discovered Quinetide markers of PQ and using ultrahigh-performance liquid chromatography-quadrupole time-of-flight mass spectrometry analysis. These "peptide markers" were validated by simultaneously monitoring Rf and F11 as standard ginsenosides.
We discovered 100 Quinetides of PQ with various post-translational modifications (PTMs), including a series of glycopeptides, all of which originated from the protein ginseng major protein. We effectively distinguished PQ from using new "peptide markers." Four unique peptides (Quinetides TP6 and TP7 as markers of PQ and Quinetides TP8 and TP9 as markers of ) and their associated glycosylation products were discovered in PQ and .
We provide specific information on PQ peptides and propose the clinical application of peptide markers to distinguish PQ from .
肽在植物中具有多种重要的生理作用，是物种鉴定的理想标志物。尚不清楚西洋参（PQ）中是否存在特定肽。本研究的目的是从PQ中鉴定人参肽（Quinetides），这是一系列对核糖核酸酶样储存蛋白（人参主要蛋白）进行不同翻译后修饰的天然肽，以探索新型肽标志物，并开发一种区分PQ与[未提及的对象]的新方法。
我们在LTQ Orbitrap分析中使用不同的碎裂模式来鉴定PQ中富集的人参肽靶点，并通过超高效液相色谱 - 四极杆飞行时间质谱分析发现了PQ和[未提及的对象]的人参肽标志物。这些“肽标志物”通过同时监测Rf和F11作为标准人参皂苷进行验证。
我们发现了100种具有各种翻译后修饰（PTM）的PQ人参肽，包括一系列糖肽，它们均源自人参主要蛋白。我们使用新的“肽标志物”有效地将PQ与[未提及的对象]区分开来。在PQ和[未提及的对象]中发现了四种独特的肽（人参肽TP6和TP7作为PQ的标志物，人参肽TP8和TP9作为[未提及的对象]的标志物）及其相关的糖基化产物。
我们提供了有关PQ肽的具体信息，并提出了肽标志物在区分PQ与[未提及的对象]方面的临床应用。
