恶臭假单胞菌 FH-1 与节杆菌 NJ-1 混合菌对莠去津的降解。
Biodegradation of Atrazine by Mixed Bacteria of Klebsiella variicola Strain FH-1 and Arthrobacter sp. NJ-1.
机构信息
College of Plant Protection, Jilin Agricultural University, Changchun, 130118, China.
College of Resource and Environment, Jilin Agricultural University, Changchun, 130118, China.
出版信息
Bull Environ Contam Toxicol. 2020 Sep;105(3):481-489. doi: 10.1007/s00128-020-02966-y. Epub 2020 Sep 10.
The purpose of this study is to enhance the biodegradability of atrazine with FH-1 and NJ-1 alone by selecting the mixing ratio, optimizing the culture medium and conditions. The results showed that FH-1 and NJ-1 have the best biodegradation effect on atrazine being mixed in a volume ratio of 3:2. In a single factor experiment, sucrose and NHCl provided carbon and nitrogen sources for the mixed bacteria. Subsequently, composition of fermentation medium was further optimized using Box-Behnken design of response surface methodology. Based on the results, growth of mixed bacteria and biodegradation of atrazine performed best effects with a biodegradation rate of 85.6% when sucrose and NHCl amounts were 35.30 g/L and 10.28 g/L. The optimal medium condition was 10% inoculum of mixed bacteria, with initial atrazine concentration of 50 mg/L, neutral or weakly alkaline pH value, 30°C. The biodegradation rate reached 97.4%, 11.8% higher than the unoptimized condition.
本研究旨在通过选择混合比例、优化培养基和条件来提高莠去津的生物降解性。结果表明，FH-1 和 NJ-1 对体积比为 3:2 的莠去津混合具有最佳的生物降解效果。在单因素实验中，蔗糖和 NHCl 为混合菌提供了碳源和氮源。随后，采用响应面法的 Box-Behnken 设计进一步优化了发酵培养基的组成。基于实验结果，当蔗糖和 NHCl 用量分别为 35.30 g/L 和 10.28 g/L 时，混合菌的生长和莠去津的生物降解效果最佳，生物降解率达到 85.6%。最佳培养基条件为混合菌接种量 10%，初始莠去津浓度 50 mg/L，中性或弱碱性 pH 值，30°C。生物降解率达到 97.4%，比未优化条件下提高了 11.8%。
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