Department of Chemical Engineering, Delft University of Technology, van der Maasweg 9, Delft, 2629 HZ, The Netherlands.
Department of Chemical Engineering, East China University of Science and Technology, Meilong 130, Shanghai, 200237, P. R. China.
Angew Chem Int Ed Engl. 2020 Dec 21;59(52):23748-23754. doi: 10.1002/anie.202009701. Epub 2020 Oct 25.
Reported here is a 2D, interfacial microcompartmentalization strategy governed by 3D phase separation. In aqueous polyethylene glycol (PEG) solutions doped with biotinylated polymers, the polymers spontaneously accumulate in the interfacial layer between the oil-surfactant-water interface and the adjacent polymer phase. In aqueous two-phase systems, these polymers first accumulated in the interfacial layer separating two polymer solutions and then selectively migrated to the oil-PEG interfacial layer. By using polymers with varying photopolymerizable groups and crosslinking rates, kinetic control and capture of spatial organisation in a variety of compartmentalized macroscopic structures, without the need of creating barrier layers, was achieved. This selective interfacial accumulation provides an extension of 3D phase separation towards synthetic compartmentalization, and is also relevant for understanding intracellular organisation.
本文报道了一种受 3D 相分离控制的 2D 界面微区化策略。在含有生物素化聚合物的水相聚乙二醇（PEG）溶液中，聚合物会自发地在油-表面活性剂-水界面与相邻聚合物相之间的界面层中聚集。在双水相体系中，这些聚合物首先在分离两种聚合物溶液的界面层中聚集，然后选择性地迁移到油-PEG 界面层。通过使用具有不同光聚合基团和交联速率的聚合物，可以实现对各种分隔宏观结构中空间组织的动力学控制和捕获，而无需创建阻挡层。这种选择性的界面聚集为合成分隔化提供了对 3D 相分离的扩展，也与细胞内组织的理解有关。
