Sakanari J A, Loinaz H M, Deardorff T L, Raybourne R B, McKerrow J H, Frierson J G
Department of Pathology, University of California, San Francisco 94143.
Am J Clin Pathol. 1988 Jul;90(1):107-13. doi: 10.1093/ajcp/90.1.107.
The diagnosis of the fourth reported case of intestinal anisakiasis in the United States was based on the morphologic characteristics of the worm in histologic sections of resected ileum. Detection of antibodies to the worm by a radioallergosorbent test (RAST), enzyme-linked immunosorbent assay (ELISA), and an immunofluorescent antibody assay (IFA) support the diagnosis. IgE antibodies in patient serum reacted specifically to larval Anisakis antigens but not to larval Ascaris antigens in the RAST. IgG and IgM antibodies to larval Anisakis antigen could be detected by ELISA up to six months after infection. IFA findings demonstrated that patient serum recognized the excretory-secretory products produced by the worm and showed their presence in vivo. These products appear to originate from the excretory pore and dorsal esophageal gland of the larval parasite.
美国第四例肠道异尖线虫病的诊断基于切除回肠组织切片中蠕虫的形态学特征。通过放射变应原吸附试验（RAST）、酶联免疫吸附测定（ELISA）和免疫荧光抗体测定（IFA）检测针对该蠕虫的抗体，这些检测结果支持了诊断。在RAST中，患者血清中的IgE抗体与异尖线虫幼虫抗原发生特异性反应，但不与蛔虫幼虫抗原发生反应。感染后长达六个月，ELISA均可检测到针对异尖线虫幼虫抗原的IgG和IgM抗体。IFA结果表明，患者血清识别该蠕虫产生的排泄-分泌产物，并显示这些产物在体内的存在。这些产物似乎源自幼虫寄生虫的排泄孔和背侧食管腺。
