Desowitz R S, Raybourne R B, Ishikura H, Kliks M M
Trans R Soc Trop Med Hyg. 1985;79(2):256-9. doi: 10.1016/0035-9203(85)90351-7.
The serum of patients with parasitologically confirmed and one patient with a clinically presumptive case of anisakiasis were tested by the radioallergosorbent test (RAST) and counter-electrophoresis (CEP) for IgE and IgG antibodies, respectively, using antigens prepared from larval (L3) stage Anisakis simplex and larval (L2) stage Toxocara canis. All sera were RAST-positive to the A. simplex antigen and RAST-negative to the T. canis antigen. All sera were CEP-negative to both antigens. The presence of a specific IgE antibody suggests that the RAST could serve as a useful technique for the serodiagnosis of anisakiasis in man.
对经寄生虫学确诊的异尖线虫病患者的血清以及 1 例临床疑似异尖线虫病患者的血清，分别采用放射性变应原吸附试验（RAST）和对流免疫电泳（CEP）检测 IgE 和 IgG 抗体，所用抗原分别由简单异尖线虫幼虫（L3）期和犬弓首线虫幼虫（L2）期制备。所有血清对简单异尖线虫抗原 RAST 呈阳性，对犬弓首线虫抗原 RAST 呈阴性。所有血清对两种抗原 CEP 均呈阴性。特异性 IgE 抗体的存在表明，RAST 可作为人类异尖线虫病血清诊断的一种有用技术。
