Department of Microbiology and Immunology, Faculty of Pharmacy, Al-Azhar University, Assuit 71524, Egypt.
Department of Microbiology and Immunology, Faculty of Pharmacy, El-Minia University, Minia 61111, Egypt.
J Glob Antimicrob Resist. 2020 Dec;23:211-216. doi: 10.1016/j.jgar.2020.08.003. Epub 2020 Sep 9.
This study aimed to determine the prevalence of virulence factors among uropathogenic Escherichia coli (UPEC) isolates from cancer patients and to investigate their genetic diversity using ERIC-PCR.
A total of 42 E. coli were recovered from urine samples from cancer patients admitted to Assiut University Hospital. PCR was used to detect the presence of three virulence genes (papC, iutA and cnf1). Genetic diversity of the isolates was determined using the ERIC-PCR fingerprinting method, and amplified products were separated by agarose gel electrophoresis. Gel electrophoresis banding patterns were used for dendrogram generation using NTSYSpc software.
Among the 42 UPEC isolates, papC was the most common virulence gene (55% of isolates), followed by iutA (38%) and cnf1 (2%). ERIC-PCR successfully produced multiple amplicons (range 2-11 bands) in each strain, with molecular weights ranging from 285 to 3000 bp. Some UPEC isolates had identical ERIC-PCR profiles (identical banding patterns), whilst 22 UPEC isolates had different ERIC-PCR profiles. The phylogenetic dendrogram of ERIC-PCR showed that the 42 isolates can be differentiated into three major clusters (I, II and III), with cluster I representing 76% of isolates, cluster II representing 19% and cluster III representing 5%.
The results of this study suggest that both papC and iutA genes may have an important role in the pathogenesis of overt urinary tract infection. Dendrogram analysis of the ERIC-PCR profiles revealed that all UPEC isolates were assigned into three main clusters, indicating the spread of distinct clonal groups that are responsible for hospital-acquired infections.
本研究旨在确定癌症患者尿路致病性大肠杆菌（UPEC）分离株中毒力因子的流行情况，并采用 ERIC-PCR 检测其遗传多样性。
从入住 Assiut 大学医院的癌症患者尿液样本中回收了 42 株大肠杆菌。采用 PCR 检测 3 种毒力基因（papC、iutA 和 cnf1）的存在情况。采用 ERIC-PCR 指纹图谱法测定分离株的遗传多样性，用琼脂糖凝胶电泳分离扩增产物。采用 NTSYSpc 软件对凝胶电泳带型进行聚类分析。
在 42 株 UPEC 分离株中，papC 是最常见的毒力基因（55%的分离株），其次是 iutA（38%）和 cnf1（2%）。ERIC-PCR 成功地在每个菌株中产生了多个扩增子（范围为 2-11 个条带），分子量范围为 285-3000bp。一些 UPEC 分离株具有相同的 ERIC-PCR 图谱（相同的带型），而 22 株 UPEC 分离株具有不同的 ERIC-PCR 图谱。ERIC-PCR 的系统发育树状图显示，42 株分离株可分为三个主要簇（I、II 和 III），I 簇占 76%，II 簇占 19%，III 簇占 5%。
本研究结果表明，papC 和 iutA 基因可能在显性尿路感染的发病机制中起重要作用。ERIC-PCR 图谱的聚类分析表明，所有 UPEC 分离株均被分为三个主要簇，表明负责医院获得性感染的不同克隆群的传播。
