Institute of Fermentation Technology and Microbiology, Department of Biotechnology and Food Sciences, Lodz University of Technology, Wólczańska 171/173, 90-924 Łódź, Poland.
Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego 48, 60-627 Poznań, Poland.
Toxins (Basel). 2020 Sep 9;12(9):578. doi: 10.3390/toxins12090578.
The feed supplementation of probiotic microorganisms is a promising method for detoxification of ochratoxin A (OTA) in poultry. The aim of the study was to investigate the effect of newly elaborated synbiotics on the turkey performance, the intestinal microbiota and its enzymatic activity in turkeys (0-15 weeks) fed OTA contaminated feed (198.6-462.0 µg/kg) compared to control group (OTA-free feed). The studies determined the composition of intestinal microorganisms by the culture method and the activity of fecal enzymes by spectrophotometry. It was found that OTA had an adverse effect on the body weight, the intestinal microbiota and the fecal enzymes activity in turkeys. On the other hand, synbiotics resulted in an increase in the count of beneficial bacteria while reducing the number of potential pathogens in the digestive tract. Moreover, synbiotics caused an increase in the activity of α-glucosidase and α-galactosidase, while decreasing the activity of potentially harmful fecal enzymes (β-glucosidase, β-galactosidase, β-glucuronidase) in the turkey's excreta. Results indicate a beneficial effect of elaborated synbiotics on the health of turkeys and a reduction of the negative impact of OTA contaminated feed. These synbiotics can be successfully used as feed additives for turkeys.
在禽类中，添加益生菌微生物作为解毒剂来去除黄曲霉毒素 A（OTA）是一种很有前景的方法。本研究的目的是为了研究新型合生元对感染 OTA （198.6-462.0 µg/kg）污染饲料的火鸡（0-15 周龄）的生长性能、肠道微生物及其酶活性的影响，并与对照组（无 OTA 污染的饲料）进行对比。本研究通过培养法确定了肠道微生物的组成，通过分光光度法确定了粪便酶的活性。结果发现，OTA 对火鸡的体重、肠道微生物和粪便酶活性有不良影响。另一方面，合生元增加了有益细菌的数量，同时减少了消化道中潜在病原体的数量。此外，合生元还提高了 α-葡萄糖苷酶和 α-半乳糖苷酶的活性，降低了火鸡粪便中潜在有害酶（β-葡萄糖苷酶、β-半乳糖苷酶、β-葡糖醛酸酶）的活性。结果表明，新型合生元对火鸡的健康有有益的影响，并降低了 OTA 污染饲料的负面影响。这些合生元可以成功地用作火鸡的饲料添加剂。
