Laboratory of Molecular Neurobiology, Center for Biomedical Research of La Rioja (CIBIR), 26006 Logroño, Spain.
MITOX Group, Department of Pharmacology and Toxicology, Faculty of Pharmacy, Universidad de Navarra, 31008 Pamplona, Spain.
Toxins (Basel). 2021 Feb 1;13(2):106. doi: 10.3390/toxins13020106.
Gut microbiota plays crucial roles in maintaining host health. External factors, such as diet, medicines, and environmental toxins, influence the composition of gut microbiota. Ochratoxin A (OTA) is one of the most prevalent and relevant mycotoxins and is a highly abundant food and animal feed contaminant. In the present study, we aimed to investigate OTA gut microbiome toxicity in mice sub-chronically exposed to low doses of OTA (0.21, 0.5, and 1.5 mg/kg body weight) by daily oral gavage for 28 days. Fecal microbiota from control and OTA-treated mice was analyzed using 16S ribosomal RNA (rRNA) gene sequencing followed by metagenomics. OTA exposure caused marked changes in gut microbial community structure, including the decrease in the diversity of fecal microbiota and the relative abundance of , as well as the increase in the relative abundance of at the phylum level. At the family level, six bacterial families (unclassified unclassified ) were significantly altered by OTA exposure. Interestingly, OTA-induced changes were observed in the lower-dose OTA groups, while high-dose OTA group microbiota was similar to control group. Our results demonstrated that sub-chronic exposure at low doses of OTA alters the structure and diversity of the gut microbial community.
肠道微生物群在维持宿主健康方面发挥着至关重要的作用。饮食、药物和环境毒素等外部因素影响肠道微生物群的组成。赭曲霉毒素 A（OTA）是最常见和相关的霉菌毒素之一，也是一种高丰度的食物和动物饲料污染物。在本研究中，我们旨在通过每日口服灌胃 28 天，研究低剂量 OTA（0.21、0.5 和 1.5mg/kg 体重）慢性暴露对小鼠肠道微生物组的毒性。通过 16S 核糖体 RNA（rRNA）基因测序和宏基因组学分析来自对照和 OTA 处理的小鼠的粪便微生物群。OTA 暴露导致肠道微生物群落结构发生明显变化，包括粪便微生物群多样性降低和相对丰度降低，以及门水平相对丰度增加。在科水平上，有六个细菌科（未分类的未分类 ）被 OTA 暴露显著改变。有趣的是，在低剂量 OTA 组中观察到 OTA 诱导的变化，而高剂量 OTA 组的微生物群与对照组相似。我们的研究结果表明，低剂量 OTA 的慢性暴露改变了肠道微生物群落的结构和多样性。
