Ortiz-Santos Elvis, Valdés-Ramírez Gabriela, Millán-Pacheco Cesar, Serratos Iris N, Lozano-Camargo Maria Luisa, Dalmasso Pablo, Rivas Gustavo A, Galicia Laura
Departamento de Química, Universidad Autónoma Metropolitana Unidad Iztapalapa (UAM-I), Av. San Rafael Atlixco 186, Leyes de Reforma 1ra Secc., Mexico City 09340, Mexico.
Facultad de Farmacia, Universidad Autónoma del Estado de Morelos (UAEM), Cuernavaca 62209, Mexico.
Biosensors (Basel). 2025 Apr 29;15(5):279. doi: 10.3390/bios15050279.
This work presents the development of a biosensing platform for hydrogen peroxide (HO) electrochemical reduction. The developed platform uses a multi-walled carbon nanotube paste (PMWCNT) and the enzyme cholesterol oxidase (ChOx). The supramolecular architecture of the PMWCNT/ChOx platform was characterized using cyclic voltammetry, electrochemical impedance spectroscopy, and amperometry. The results indicated that the presence of ChOx enhances the sensitivity of electrochemical detection for HO by 21 times compared to that without ChOx. The designed electrochemical sensing bio-platform for HO shows a sensitivity of 26.15 µA/mM in the linear range from 0.4 to 4.0 mM, an LOD of 0.43 µM, and an LOQ of 1.31 µM. Furthermore, in silico studies (molecular dynamics simulations, molecular docking assays, and binding free energy calculations (Δ)) were carried out to characterize and validate the molecular interaction between ChOx and HO. The computed data confirmed that the binding is spontaneous, and the type of labile interaction promotes a rapid electrochemical reduction of HO.
这项工作展示了一种用于过氧化氢（HO）电化学还原的生物传感平台的开发。所开发的平台使用多壁碳纳米管糊剂（PMWCNT）和胆固醇氧化酶（ChOx）。采用循环伏安法、电化学阻抗谱和安培法对PMWCNT/ChOx平台的超分子结构进行了表征。结果表明，与没有ChOx的情况相比，ChOx的存在使HO电化学检测的灵敏度提高了21倍。所设计的用于HO的电化学传感生物平台在0.4至4.0 mM的线性范围内灵敏度为26.15 µA/mM，检测限为0.43 µM，定量限为1.31 µM。此外，还进行了计算机模拟研究（分子动力学模拟、分子对接分析和结合自由能计算（Δ）），以表征和验证ChOx与HO之间的分子相互作用。计算数据证实这种结合是自发的，不稳定相互作用的类型促进了HO的快速电化学还原。
