Department of Physiology, Brazil; Graduate Program of Health Sciences. Federal University of Sergipe, São Cristóvão, SE, 49100-000, Brazil.
Department of Physiology, Brazil.
J Ethnopharmacol. 2021 Jan 30;265:113338. doi: 10.1016/j.jep.2020.113338. Epub 2020 Sep 10.
Medicinal plants remain an invaluable source for therapeutics of diseases that affect humanity. Sideritis bilgeriana (Lamiaceae) is medicinal plant used in Turkey folk medicine to reduce inflammation and pain, but few studies scientific corroborates its medicinal use so creating a gap between popular use and scientific evidence. Thus, we aimed to evaluate the pharmacological effects of the methanolic extract of S. bilgeriana (MESB) in rodents nociception models and also performed its phytochemical analysis. Firstly, a screening was carried out that enabled the identification of the presence of phenolic compounds and flavonoids. In view of this, a chromatographic method by HPLC-DAD-UV was developed that made it possible to identify chlorogenic acid and its quantification in MESB. MESB-treated mice (MESB 50, 100 and 200 mg/kg, p.o.) reduced mechanical hyperalgesia and myeloperoxidase activity (p < 0.01), and also showed a reduced pain behavior in capsaicin test. In the carrageenan-induced pleurisy test, MESB (100 mg/kg p.o.) significantly reduced the leukocyte (polymorphonuclear) count in the pleural cavity and equally decreased the TNF-α and IL-1β levels (p < 0.001). In the PSNL model, mechanical hyperalgesia was reduced on the first evaluation day and during the 7 days of evaluation compared to the vehicle group (p < 0.001). Thermal hyperalgesia was also reduced 1 h after treatment compared to the vehicle group (p < 0.001) and reversed the loss of force initially displayed by the animals, thus inferring an analgesic effect in the muscle strength test. Analysis of the marrow of these animals showed a decrease in the level of pro-inflammatory cytokine IL-6 (p < 0.001) and factor NF-κB, in relation to the control group (p < 0.05). Moreover, the MESB treatment produced no noticeable side effects, no disturb in motor performance and no signs of gastric or hepatic injury. Together, the results suggests that MESB could be useful to management of inflammation and neuropathic pain mainly by the management of pro-inflammatory mediators (NF-κB, TNF-α, IL-1β and IL-6), so reinforcing its use in popular medicine and corroborating the need for further chemical and pharmacological studies for the species.
药用植物仍然是治疗影响人类疾病的宝贵资源。在土耳其民间医学中，Sideritis bilgeriana（唇形科）被用作一种药物，用于减轻炎症和疼痛，但很少有科学研究证实其药用价值，因此在民间应用和科学证据之间存在差距。因此，我们旨在评估 S. bilgeriana（MESB）的甲醇提取物在啮齿动物疼痛模型中的药理作用，并对其进行植物化学分析。首先，进行了筛选，以确定是否存在酚类化合物和类黄酮。有鉴于此，开发了一种通过 HPLC-DAD-UV 进行的色谱方法，该方法可以鉴定绿原酸并对其进行定量分析。MESB 治疗的小鼠（MESB 50、100 和 200mg/kg，口服）减轻了机械性痛觉过敏和髓过氧化物酶活性（p<0.01），并且在辣椒素测试中也表现出疼痛行为减少。在角叉菜胶诱导的胸膜炎试验中，MESB（100mg/kg 口服）显著减少了胸膜腔中的白细胞（多形核）计数，并同样降低了 TNF-α和 IL-1β的水平（p<0.001）。在 PSNL 模型中，与载体组相比，在第一个评估日和 7 天的评估期间，机械性痛觉过敏减少（p<0.001）。与载体组相比，1 小时后治疗还减轻了热痛觉过敏（p<0.001），并逆转了动物最初表现出的力量损失，从而推断出肌肉力量测试中的镇痛作用。对这些动物的骨髓分析表明，与对照组相比（p<0.05），促炎细胞因子 IL-6 的水平降低（p<0.001）和 NF-κB 因子。此外，MESB 治疗没有产生明显的副作用，没有运动表现的干扰，也没有胃或肝损伤的迹象。综上所述，结果表明 MESB 可能对炎症和神经病理性疼痛的管理有用，主要是通过管理促炎介质（NF-κB、TNF-α、IL-1β和 IL-6），因此加强了其在民间医学中的应用，并证实了对该物种进行进一步的化学和药理学研究的必要性。
