Department of Horticultural Sciences, Cape Peninsula University of Technology, Symphony Rd., Bellville 7535, South Africa.
Chemistry Department, Cape Peninsula University of Technology, Symphony Rd., Bellville 7535, South Africa.
Molecules. 2020 Sep 10;25(18):4129. doi: 10.3390/molecules25184129.
The prevalence of diabetes mellitus (DM), considered one of the most common metabolic disorders, has dramatically increased and resulted in higher rates of morbidity and mortality around the world in the past decade. It is well known that insulin resistance in target tissues and a deficiency in insulin secretion from pancreatic β-cells are the main characteristics of type 2 diabetes. The aim of this study was the bio-evaluation of compounds isolated from three selected plant species: namely, , , and , for their glucose-uptake ability. Methanolic extracts were produced from the aerial parts of each plant. Compounds were identified using different spectroscopic techniques. The glucose-uptake ability of each compound was then evaluated in mammalian cells using 2-deoxyglucose-6-phosphate. The cytotoxicity of each compound was established via the MTT assay. Chromatographic purification of the three plant species yielded sixteen pure terpenoids. Compounds ( = 0.0031), ( = 0.0053), and ( = 0.0086) showed a marked increase in glucose uptake, respectively. Additionally, , , and exhibited cytotoxicity toward mammalian tissue with a decrease in cell viability of ~70%, ~68%, and ~67%, respectively. The results suggested that several compounds demonstrated a marked increase in glucose uptake, while two of the compounds exhibited signs of cytotoxicity. It may, therefore, be suggested that these compounds be considered as potential candidates for novel plant-derived alternative therapies in the treatment of type 2 diabetes.
糖尿病（DM）的患病率被认为是最常见的代谢紊乱之一，在过去十年中在全球范围内急剧增加，并导致发病率和死亡率上升。众所周知，靶组织中的胰岛素抵抗和胰腺β细胞中胰岛素分泌的不足是 2 型糖尿病的主要特征。本研究的目的是从三种选定的植物物种中分离的化合物的生物评估：即、和，用于其葡萄糖摄取能力。从每种植物的地上部分生产甲醇提取物。使用不同的光谱技术鉴定化合物。然后使用 2-脱氧葡萄糖-6-磷酸在哺乳动物细胞中评估每种化合物的葡萄糖摄取能力。通过 MTT 测定法确定每种化合物的细胞毒性。三种植物物种的色谱纯化得到了十六种纯萜类化合物。化合物（= 0.0031）、（= 0.0053）和（= 0.0086）分别显示出葡萄糖摄取的明显增加。此外，、和对哺乳动物组织表现出细胞毒性，细胞活力分别降低了约 70%、68%和 67%。结果表明，几种化合物显示出明显的葡萄糖摄取增加，而两种化合物表现出细胞毒性迹象。因此，可以认为这些化合物可被视为新型植物衍生替代疗法治疗 2 型糖尿病的潜在候选物。
