Animal Care Services, University of Western Australia, Perth, Western Australia, Australia;, Email:
Animal Care Services, University of Western Australia, Perth, Western Australia, Australia.
J Am Assoc Lab Anim Sci. 2020 Nov 1;59(6):732-736. doi: 10.30802/AALAS-JAALAS-20-000027. Epub 2020 Sep 14.
The purpose of this study was to compare the safety and efficacy of buffered tricaine methanesulfonate (MS-222) and isoeugenol for the anesthesia of zebrafish undergoing caudal fin clipping. Eighty 9 mo (AB strain) zebrafish were allocated to one of 2 equal groups: buffered MS-222 (168 mg/L, = 40) or isoeugenol (20 mg/L, = 40). The time to induction of anesthesia was significantly shorter in the isoeugenol group (141 ± 70 s) than in the MS-222 group (207 ± 103 s). The time to recovery from anesthesia was also shorter in the MS-222 group (373 ± 125 s) than in the isoeugenol group (491 ± 176 s). No obvious displays of distress or aversion to anesthesia were observed in either group. No difference was detected in the proportion of zebrafish that became anesthetized with either drug. One male zebrafish in the buffered MS-222 group was found dead at the 1-h post-procedural monitoring time point, but there was no difference between groups in the proportion of fish that survived anesthesia to the end of experiment. In conclusion, the safety and efficacy of buffered MS-222 (168 mg/L) and isoeugenol (20 mg/L) was similar for zebrafish undergoing anesthesia for caudal fin clipping.
本研究旨在比较丁卡因盐酸盐（MS-222）和异丁香酚在斑马鱼尾鳍修剪麻醉中的安全性和有效性。将 80 尾 9 月龄（AB 品系）斑马鱼随机分为两组，每组 40 尾：缓冲 MS-222（168mg/L）或异丁香酚（20mg/L）。异丁香酚组麻醉诱导时间（141±70s）显著短于 MS-222 组（207±103s）。MS-222 组麻醉恢复期也短于异丁香酚组（373±125s）。两组均未见明显的麻醉不适或麻醉抗拒表现。两种药物麻醉的斑马鱼比例无差异。MS-222 缓冲液组 1 尾雄性斑马鱼在术后 1 小时监测时间点死亡，但两组麻醉至实验结束的鱼存活率无差异。结论：用于斑马鱼尾鳍修剪麻醉时，缓冲 MS-222（168mg/L）和异丁香酚（20mg/L）的安全性和有效性相似。
