School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, 510006, China.
School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, 510006, China; Key Laboratory of State Administration of TCM for Digital Quality Evaluation of Chinese Materia Medica, Guangzhou, 510006, China; Engineering &Technology Research Center for Chinese Materia Medica Quality of the Universities of Guangdong Province, Guangzhou, 510006, China.
Talanta. 2020 Dec 1;220:121368. doi: 10.1016/j.talanta.2020.121368. Epub 2020 Jul 10.
A new method for fishing antitumor ingredients by G-quadruplex recognition from Macleaya cordata seeds extracts was established using a three-phase-laminar-flow-chip (TPL chip). The TPL chip integrated the separation of drugs from the complex ingredients and organic solvent extraction, simplifying pretreatment processes and reducing reagents and time. In addition, the chip method showed a lower false negative result, owing to the gentle membrane-free filtration process based on diffusion separation in microchannel. Four ligands with high content in alkaloids of Macleaya cordata seeds were selected, those are chelerythrine (CHE), sanguinarine (SAN), protopine (PRO), and allocryptopine (ALL), which demonstrated affinity with G-quadruplex and were potential for antitumor.
建立了一种利用三层层流芯片（TPL 芯片）从山梗菜种子提取物中识别 G-四链体来提取抗肿瘤成分的新方法。TPL 芯片集成了从复杂成分中分离药物和有机溶剂提取，简化了预处理过程，减少了试剂和时间。此外，由于基于微通道扩散分离的无膜过滤过程温和，该芯片方法显示出较低的假阴性结果。从山梗菜种子的生物碱中选择了四种含量较高的配体，即白屈菜红碱（CHE）、血根碱（SAN）、原阿片碱（PRO）和阿罗克林（ALL），它们与 G-四链体具有亲和力，是潜在的抗肿瘤药物。
