Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, Changsha 410081, China.
Laboratory of Organic Chemistry, Wageningen University, Stippeneng 4, 6708 WE Wageningen, The Netherlands.
Molecules. 2020 Dec 23;26(1):38. doi: 10.3390/molecules26010038.
Induced phase separation extraction (IPSE) is an efficient sample clean-up technique that can replace liquid-liquid extraction (LLE). The purpose of this study was to miniaturize IPSE by carrying it out in a microfluidic chip. An IPSE chip was designed and evaluated for its ability to separate and purify samples on a microscale. The 5 × 2 cm chip was fed with a solution of polar to non-polar model compounds in acetonitrile-water (1:1). In the 100 µm wide and 40 µm deep microchannels, the sample solution was efficiently separated into two immiscible phases by adding a hydrophobic solvent as inducer. Analytes present in the sample solution each migrated to their own favorable phase upon phase separation. After optimization, extraction and fractionation were easily and efficiently achieved. The behavior of analytes with a pH-dependent partitioning could be influenced by adjusting the pH of the sample solution. extract, used in Traditional Chinese Medicine (TCM), was successfully separated in aglycones and glycosides. In this microscale system, the sample and solvent consumption is reduced to microliters, while the time needed for the sample pretreatment is less than one minute. Additionally, the extraction efficiency can reach up to 98.8%, and emulsion formation is avoided.
诱导相分离萃取（IPSE）是一种高效的样品净化技术，可以替代液液萃取（LLE）。本研究旨在通过在微流控芯片中进行 IPSE 来实现微型化。设计并评估了 IPSE 芯片，以评估其在微尺度上分离和净化样品的能力。该 5×2 厘米的芯片用极性到非极性模型化合物在乙腈-水（1:1）中的溶液进料。在 100µm 宽和 40µm 深的微通道中，通过添加疏溶剂作为诱导剂，可有效地将样品溶液分离成两个不混溶的相。样品溶液中的分析物在相分离时各自迁移到其自身有利的相中。经过优化，很容易实现萃取和分级。可以通过调整样品溶液的 pH 值来影响具有 pH 依赖性分配的分析物的行为。在这种微尺度系统中，样品和溶剂的消耗减少到微升，而样品预处理所需的时间不到一分钟。此外，萃取效率可高达 98.8%，并且避免了乳状液的形成。中药中使用的甘草酸成功地分离为苷元和糖苷。
