Wang Lei, Ling Yu, Han Lei, Zhou Jiao, Sun Zhe, Li Nian Bing, Luo Hong Qun
Key Laboratory of Eco-Environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, China.
Key Laboratory of Eco-Environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, China.
Anal Chim Acta. 2020 Sep 22;1131:118-125. doi: 10.1016/j.aca.2020.07.051. Epub 2020 Jul 27.
Mimic enzymes greatly improve the inherent insufficiencies of natural enzymes. Therefore, mimic enzyme sensors attract increasing research interest. Metal-organic framework (MOF) is emerging in the field of mimic enzyme catalysis due to its remarkable structural properties. In this paper, a colorimetric method is designed for rapid and sensitive detection of glucose and cysteine levels. The MOF Eu-pydc (pydc-2,5-pyridinedicarboxylic acid) is synthesized by a new strategy which is regulated by ligands at room temperature and found to have peroxidase activity. Then, the MOF is used as a mimic enzyme to catalyze chromogenic substrate (3,3',5,5'-tetramethylbenzidine, TMB) for colorimetric sensing of glucose. The developed method can accurately detect glucose in the range of 10 μM-1 mM (R = 0.9958) with a relatively low detection limit about 6.9 μM. Moreover, a cysteine sensor with a detection limit of 0.28 μM is also established based on the disappearance of the color of oxTMB. Additionally, the proposed glucose sensor exhibits excellent selectivity and is successfully applied to blood glucose detection. At the same time, the detection of cysteine is also highly sensitive. In short, the dual sensor is fast, low cost, and convenient, and has great application potential in the diagnosis of disease.
模拟酶极大地改善了天然酶固有的不足。因此，模拟酶传感器吸引了越来越多的研究兴趣。金属有机框架（MOF）由于其卓越的结构特性而在模拟酶催化领域崭露头角。本文设计了一种比色法用于快速、灵敏地检测葡萄糖和半胱氨酸水平。通过一种由配体在室温下调控的新策略合成了MOF Eu-pydc（pydc-2,5-吡啶二甲酸），并发现其具有过氧化物酶活性。然后，将该MOF用作模拟酶来催化显色底物（3,3',5,5'-四甲基联苯胺，TMB）用于葡萄糖的比色传感。所开发的方法能够在10 μM - 1 mM范围内准确检测葡萄糖（R = 0.9958），检测限相对较低，约为6.9 μM。此外，基于氧化型TMB颜色消失还建立了检测限为0.28 μM的半胱氨酸传感器。此外，所提出的葡萄糖传感器具有出色的选择性，并成功应用于血糖检测。同时，对半胱氨酸的检测也具有高度敏感性。简而言之，该双传感器快速、低成本且方便，在疾病诊断中具有巨大的应用潜力。
