School of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao 266033, China.
Research Center for Intelligent and Wearable Technology, College of Textiles and Clothing, State Key Laboratory of Bio-Fibers and Eco-Textiles, Qingdao University, Qingdao 266071, China.
Anal Chem. 2023 Jul 18;95(28):10785-10794. doi: 10.1021/acs.analchem.3c01905. Epub 2023 Jul 10.
A novel metal-organic framework (MOF)-on-MOF dual enzyme-mimic nanozyme was designed as enhanced cascade signal amplification for the colorimetric and chemiluminescent (CL) dual-mode aptasensing. The MOF-on-MOF hybrid is composed of MOF-818 with catechol oxidase-like activity and iron porphyrin MOF [PMOF(Fe)] with peroxidase-like activity, called MOF-818@PMOF(Fe). MOF-818 can catalyze the 3,5-di--butylcatechol substrate and produce HO in situ. Subsequently, PMOF(Fe) catalyzes HO to produce reactive oxygen species, which oxidize 3,3',5,5'-tetramethylbenzidine or luminol to produce color or luminescence. Thanks to the nano-proximity effect and the confinement effect, the efficiency of the biomimetic cascade catalysis is greatly improved, which in turn generates enhanced colorimetric and CL signals. Taking the detection of chlopyrifos as an example, the prepared dual enzyme-mimic MOF nanozyme is combined with the aptamer with specific recognition ability to develop a colorimetric/CL dual-mode aptasensor for highly sensitive and selective detection of chlorpyrifos. The proposed MOF-on-MOF dual nanozyme-enhanced cascade system may provide a new pathway for the further development of a biomimetic cascade sensing platform.
一种新型的金属-有机骨架（MOF）-MOF 双重酶模拟纳米酶被设计为增强级联信号放大，用于比色和化学发光（CL）双模式适体传感。MOF-on-MOF 杂化由具有儿茶酚氧化酶样活性的 MOF-818 和具有过氧化物酶样活性的铁卟啉 MOF [PMOF(Fe)] 组成，称为 MOF-818@PMOF(Fe)。MOF-818 可以催化 3,5-二--丁基儿茶酚底物并原位产生 HO。随后，PMOF(Fe)催化 HO 产生活性氧，将 3,3',5,5'-四甲基联苯胺或鲁米诺氧化产生颜色或发光。由于纳米接近效应和限制效应，仿生级联催化的效率大大提高，从而产生增强的比色和 CL 信号。以检测毒死蜱为例，制备的双重酶模拟 MOF 纳米酶与具有特异性识别能力的适体结合，开发了一种比色/CL 双模式适体传感器，用于高灵敏度和选择性检测毒死蜱。所提出的 MOF-on-MOF 双重纳米酶增强级联系统可能为仿生级联传感平台的进一步发展提供新途径。
