Lin Chunhua, Du Yue, Wang Shiqi, Wang Li, Song Yonghai
Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Key Laboratory of Chemical Biology, Jiangxi Province, College of Chemistry and Chemical Engineering, Jiangxi Normal University, 99 Ziyang Road, Nanchang 330022, China.
Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Key Laboratory of Chemical Biology, Jiangxi Province, College of Chemistry and Chemical Engineering, Jiangxi Normal University, 99 Ziyang Road, Nanchang 330022, China.
Mater Sci Eng C Mater Biol Appl. 2021 Jan;118:111511. doi: 10.1016/j.msec.2020.111511. Epub 2020 Sep 17.
The work presents a novel glucose oxidase@Cu-hemin metal-organic frameworks (GOD@ Cu-hemin MOFs) with a ball-flower structure as bienzymatic catalysts for detection of glucose. The GOD@Cu-hemin MOFs exhibits great stability as compared with free horseradish peroxidase and GOD toward harsh conditions because the ball-flower-like shell of Cu-hemin MOF effectively protects from GOD. Thus, the GOD@Cu-Hemin MOFs can be used in external harsh conditions such as high temperature and acid/base. The GOD@Cu-hemin MOFs is capable of sensitive and selective detection of glucose via peroxidase-like of Cu-hemin MOFs and GOD by using 3,3',5,5'-tetramethylbenzidine (TMB) as a substrate. Under the existence of glucose, O is reduced into HO via GOD@Cu-hemin MOFs. The produced HO as well as Cu-hemin MOFs oxidize TMB into blue oxTMB which shows UV-Vis absorbance at 652. The absorption intensity of oxTMB linearly increases with the increasing concentration of glucose from 0.01 to 1.0 mM with detection limit of 2.8 μM. An integrated agarose hydrogel film (Aga/GOD@Cu-hemin MOF/TMB) sensor is rationally designed for colorimetric detection of glucose. The sensor displays a response range of 30 μM-0.8 mM with a detection limit of 0.01 mM. The result indicates that the Cu-hemin MOFs are an ideal carrier for the encapsulation of enzymes.
该研究展示了一种具有球花结构的新型葡萄糖氧化酶@铜-血红素金属有机框架（GOD@Cu-血红素MOFs）作为用于检测葡萄糖的双酶催化剂。与游离辣根过氧化物酶和葡萄糖氧化酶相比，GOD@Cu-血红素MOFs在恶劣条件下表现出极大的稳定性，因为铜-血红素MOF的球花状外壳有效地保护了葡萄糖氧化酶。因此，GOD@Cu-血红素MOFs可用于外部恶劣条件，如高温和酸/碱环境。GOD@Cu-血红素MOFs能够通过以3,3',5,5'-四甲基联苯胺（TMB）为底物，利用铜-血红素MOFs和葡萄糖氧化酶的过氧化物酶样活性灵敏且选择性地检测葡萄糖。在葡萄糖存在的情况下，氧气通过GOD@Cu-血红素MOFs被还原为过氧化氢。产生的过氧化氢以及铜-血红素MOFs将TMB氧化为蓝色的氧化型TMB，其在652处呈现紫外可见吸收。氧化型TMB的吸收强度随着葡萄糖浓度从0.01到1.0 mM的增加而线性增加，检测限为2.8 μM。一种集成的琼脂糖水凝胶膜（Aga/GOD@Cu-血红素MOF/TMB）传感器被合理设计用于葡萄糖的比色检测。该传感器的响应范围为30 μM - 0.8 mM，检测限为0.01 mM。结果表明铜-血红素MOFs是包封酶的理想载体。
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