Bornstein P, Ash J F
Proc Natl Acad Sci U S A. 1977 Jun;74(6):2480-4. doi: 10.1073/pnas.74.6.2480.
Collagen and/or procollagen was demonstrated on the surface of monolayers of fibroblasts from normal rat kidney by indirect immunofluorescence with affinity-purified antibodies to collagen. The protein was arrayed in a reticular fashion on the cell surface and, in cells attached to a substratum, was severely restricted in its ability to undergo antibody-induced translational movement in the plane of the membrane. A similar pattern was observed for fibronectin (LETS protein, fibroblast surface antigen). These macromolecules were lost when fibroblasts were dissociated and examined in suspension cultures and were not regained until after the cells were replated. On the basis of the morphological findings, and in view of the likelihood of an interaction between fibronectin and collagen, we propose that these that these proteins form a meshwork on the cell surface. This external protein meshwork may mediate a number of important cellular functions, including attachment to a substratum and other interactions with the extracellular matrix.
通过用亲和纯化的抗胶原蛋白抗体进行间接免疫荧光法，在正常大鼠肾成纤维细胞单层表面证实了胶原蛋白和/或前胶原蛋白的存在。该蛋白质以网状形式排列在细胞表面，并且在附着于基质的细胞中，其在膜平面内进行抗体诱导的平移运动的能力受到严重限制。纤连蛋白（LETS蛋白，成纤维细胞表面抗原）也观察到类似的模式。当成纤维细胞解离并在悬浮培养物中检查时，这些大分子会丢失，直到细胞重新接种后才会重新获得。基于形态学发现，并鉴于纤连蛋白与胶原蛋白之间相互作用的可能性，我们提出这些蛋白质在细胞表面形成一个网络。这种外部蛋白质网络可能介导许多重要的细胞功能，包括附着于基质以及与细胞外基质的其他相互作用。
