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用单克隆抗体通过间接免疫荧光抗体试验对鹦鹉热衣原体进行免疫分型。

Immunotyping of Chlamydia psittaci by indirect immunofluorescence antibody test with monoclonal antibodies.

作者信息

Takahashi T, Takashima I, Hashimoto N

机构信息

Department of Veterinary Public Health, Faculty of Veterinary Medicine, Hokkaido University, Sapporo.

出版信息

Microbiol Immunol. 1988;32(3):251-63. doi: 10.1111/j.1348-0421.1988.tb01385.x.

DOI:10.1111/j.1348-0421.1988.tb01385.x
PMID:3292879
Abstract

Monoclonal antibodies against pigeon and budgerigar strains of Chlamydia psittaci were used to classify the immunotypes of C. psittaci strains by an indirect immunofluorescence antibody (IFA) test. Thirty-three C. psittaci strains from pigeons and 24 from budgerigars were divided into three immunotypes (P-I, P-II, and P-III) and (B-I, B-II, and B-III), respectively. Two strains from human psittacosis patients were identified as P-III and B-I, coinciding with the epidemiological evidence of each human infection. Two strains from psittacine birds, a parrot and a parakeet, were identical to the B-II immunotype. Other mammalian strains were quite distinct from avian strains in their IFA reaction with the monoclonal antibodies.

摘要

利用针对鹦鹉热衣原体鸽子株和虎皮鹦鹉株的单克隆抗体,通过间接免疫荧光抗体(IFA)试验对鹦鹉热衣原体菌株的免疫型进行分类。来自鸽子的33株鹦鹉热衣原体菌株和来自虎皮鹦鹉的24株菌株分别被分为三种免疫型(P-I、P-II和P-III)以及(B-I、B-II和B-III)。两例来自人类鹦鹉热患者的菌株被鉴定为P-III和B-I,这与每个人类感染的流行病学证据相符。来自鹦鹉科鸟类(一只鹦鹉和一只长尾小鹦鹉)的两株菌株与B-II免疫型相同。其他哺乳动物菌株在与单克隆抗体的IFA反应中与禽类菌株有很大不同。

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