Andersen A A
Avian Diseases Research Unit, National Animal Disease Center, U.S. Department of Agriculture, Ames, Iowa 50010.
J Clin Microbiol. 1991 Feb;29(2):244-9. doi: 10.1128/jcm.29.2.244-249.1991.
Avian Chlamydia psittaci isolates were examined by restriction endonuclease analysis and serovar-specific monoclonal antibodies and compared with ovine abortion and polyarthritis isolates. The avian isolates were divided into four serovars (turkey, psittacine, pigeon, and duck) based on their reactivity to the monoclonal antibodies. The DNA digest patterns were similar across the four avian serovars; most bands were identical when the isolates were tested with PstI, BamHI, and EcoRI restriction endonuclease enzymes. The turkey group restriction endonuclease analysis patterns were distinguished from those of the other avian strains by three to four band differences with all enzymes. The duck and pigeon isolates showed only minor DNA pattern differences when compared with the psittacine isolates. Four psittacine isolates from various locations in Texas had an extra band with the EcoRI restriction enzyme, suggesting that they were from a common source; however, they were indistinguishable from the other psittacine isolates when examined with the monoclonal antibodies. The avian isolates were distinctly different from either abortion or polyarthritis isolates by both restriction endonuclease analysis and monoclonal antibody analysis. The data demonstrate that the avian isolates form a distinct group or separate biovar with at least four serovars.
通过限制性内切酶分析和血清型特异性单克隆抗体对禽源鹦鹉热衣原体分离株进行了检测，并与绵羊流产和多关节炎分离株进行了比较。根据禽源分离株对单克隆抗体的反应性，将其分为四个血清型（火鸡、鹦鹉、鸽子和鸭子）。四种禽源血清型的DNA消化模式相似；当用PstI、BamHI和EcoRI限制性内切酶对分离株进行检测时，大多数条带是相同的。火鸡组的限制性内切酶分析模式与其他禽源菌株的模式不同，在所有酶的作用下有三到四条带的差异。与鹦鹉分离株相比，鸭子和鸽子分离株仅显示出微小的DNA模式差异。来自德克萨斯州不同地点的四个鹦鹉分离株在用EcoRI限制性内切酶时出现了一条额外的条带，表明它们来自同一来源；然而，在用单克隆抗体检测时，它们与其他鹦鹉分离株无法区分。通过限制性内切酶分析和单克隆抗体分析，禽源分离株与流产或多关节炎分离株明显不同。数据表明，禽源分离株形成了一个独特的组或单独的生物变种，至少有四个血清型。
