Tahtamouni Lubna Hamid, Hamdan Mahmoud Nael, Al-Mazaydeh Zainab Ali, Bawadi Randa Mahmoud, Rammaha Majdoleen Sobhi, Zghoul Ahmad Mohammad, Ahram Mamoun Ahmad, Yasin Salem Refat
Department of Biology and Biotechnology, Faculty of Science, the Hashemite University, Zarqa, Jordan.
Department of Biochemistry and Molecular Biology, College of Natural Sciences, Colorado State University, Fort Collins, Colorado, USA.
Int J Reprod Biomed. 2020 Aug 19;18(8):571-578. doi: 10.18502/ijrm.v13i8.7496. eCollection 2020 Aug.
Tissue plasminogen activator (t-PA) is a protein involved in the fibrinolytic system that catalyzes the conversion of plasminogen into the active plasmin. The activity of t-PA is controlled by plasminogen activator inhibitor-1. t-PA has crucial functions during spermatogenesis. One polymorphism was reported for gene, either the presence of a 300-bp -repeat ( ) or its absence ( ).
The current work aimed at studying the association between polymorphism in the gene and male infertility.
Using polymerase chain reaction on genomic DNA isolated from the blood of 79 participants, a region polymorphic for element insertion in gene was amplified. In addition, total t-PA concentration, plasminogen activator inhibitor-1 /t-PA complex concentration, and t-PA activity in seminal plasma were measured by enzyme-linked immunosorbent assay.
The results indicate that the percentage of infertile participants (n = 50) who were homozygous for insertion ( ), heterozygous or homozygous for deletion ( ) did not change significantly (p = 0.43, 0.81, and 0.85, respectively) when compared with the control participants (n = 29). On the other hand, a significant decrease (p = 0.0001) of t-PA total concentration in seminal plasma was observed in the infertile group in comparison with the control group. However, the results indicate that there is no association between the different genotypes and the total t-PA seminal concentration in the infertile group when compared to the control group (p = 0.63).
Data obtained from the current study does not support an association between polymorphism and t-PA seminal concentration or male infertility.
组织型纤溶酶原激活剂（t-PA）是一种参与纤维蛋白溶解系统的蛋白质，可催化纤溶酶原转化为活性纤溶酶。t-PA的活性受纤溶酶原激活剂抑制剂-1的控制。t-PA在精子发生过程中具有关键作用。该基因报道了一种多态性，即存在300bp的 -重复序列（ ）或不存在（ ）。
当前研究旨在探讨该基因多态性与男性不育之间的关联。
对从79名参与者血液中分离的基因组DNA进行聚合酶链反应，扩增该基因中 元件插入多态性的区域。此外，通过酶联免疫吸附测定法测量精浆中总t-PA浓度、纤溶酶原激活剂抑制剂-1/t-PA复合物浓度以及t-PA活性。
结果表明，与对照组（n = 29）相比，插入纯合子（ ）、杂合子 或缺失纯合子（ ）的不育参与者（n = 50）的百分比没有显著变化（分别为p = 0.43、0.81和0.85）。另一方面，与对照组相比，不育组精浆中t-PA总浓度显著降低（p = 0.0001）。然而，结果表明，与对照组相比，不育组中 不同基因型与精浆总t-PA浓度之间没有关联（p = 0.63）。
本研究获得的数据不支持 多态性与精浆t-PA浓度或男性不育之间存在关联。
