National Microbiology Laboratory-CSCHAH, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada.
Int J Syst Evol Microbiol. 2020 Nov;70(11):5676-5685. doi: 10.1099/ijsem.0.004461.
Nine Gram-stain-positive cocci, coccobacilli or short, rod-shaped strains recovered from clinical sources from patients located in two Canadian provinces and one environmental source were extensively studied. Clinical sources included blood cultures, cerebral spinal fluid, lymph node, lung biopsy and peritoneal fluid. Through 16S rRNA gene and whole genome sequencing analyses, the strains were found to cluster into three groups, closest to but distinguished from other genera in the family . The genomes from these bacteria had high G+C content, ranging from 67.8-69.56 mol%, and genome sizes of 3.02-4.52 Mb. Biochemical and chemotaxonomic properties including branched-chain cellular fatty acids, l-lysine diaminopimelic acid (ll-DAP) and cell-wall type A3γ (ll-DAP-gly) containing ll-DAP, alanine, glycine and glutamic acid were found and so the strains were therefore deemed to be consistent with other new genera in this family. Based on this investigation, we propose gen. nov., sp. nov., sp. nov. and gen. nov., sp. nov. for these taxa. Misidentified taxon '' was found to be assignable to based on this study.
从加拿大两个省的患者临床样本和一个环境样本中分离到九株革兰氏阳性球菌、球杆菌或短的、杆状的菌株,对其进行了广泛研究。临床样本包括血培养、脑脊液、淋巴结、肺活检和腹腔液。通过 16S rRNA 基因和全基因组测序分析,发现这些菌株聚类为三组,与家族中的其他属最为接近,但又有所区别。这些细菌的基因组具有较高的 G+C 含量,范围为 67.8-69.56mol%,基因组大小为 3.02-4.52Mb。发现了生化和化学分类特性,包括支链细胞脂肪酸、l-赖氨酸二氨基庚二酸(ll-DAP)和细胞壁型 A3γ(ll-DAP-gly),含有 ll-DAP、丙氨酸、甘氨酸和谷氨酸,因此这些菌株与该家族中的其他新属一致。基于这一研究,我们提出了 新属、新种、新种 和 新属、新种。本研究发现,被错误鉴定的分类单元“”可归属于 。