Grupo de Bioquímica Vegetal, Instituto de Biología Agrícola de Mendoza, Consejo Nacional de Investigaciones Científicas y Técnicas-Universidad Nacional de Cuyo, Almirante Brown 500, M5528AHB Chacras de Coria, Argentina.
Instituto de Veterinaria Ambiente y Salud, Universidad Juan A. Maza, Lateral Sur del Acceso Este 2245, 5519 Guaymallén, Argentina.
Food Chem. 2021 Feb 15;338:128030. doi: 10.1016/j.foodchem.2020.128030. Epub 2020 Sep 10.
A liquid chromatography method coupling diode-array and fluorescence detectors (DAD and FLD, respectively) has been developed for the simultaneous quantification of 32 phenolic compounds (PCs) in winemaking products. With the combination of both detectors it was possible to determine phenolic acids, flavanols, flavonols, stilbenes and other PCs in the matrices under study. An excitation wavelength of 290 nm was selected and three different emission wavelengths (315, 360 and 400 nm) were recorded. The method provided detection and quantification limits (LODs and LOQs) within the ranges of 0.01-1.46 mg/L and 0.03-4.9 mg/L, respectively. The LODs and LOQs using FLD for flavanols, stilbenes and phenyl ethanol analogues were improved between 65 and 1000% as compared with DAD. The combination of DAD with FLD increased both, sensitivity and the ability to reduce interfering signals. The developed method was applied for the characterization of PCs of wines, bunch stems and grape canes.
一种液相色谱法，结合二极管阵列和荧光检测器（分别为 DAD 和 FLD），已被开发用于同时定量检测酿造产品中的 32 种酚类化合物（PCs）。通过结合这两种检测器，可以在研究的基质中测定酚酸、黄烷醇、黄酮醇、芪类和其他 PCs。选择激发波长为 290nm，并记录三个不同的发射波长（315、360 和 400nm）。该方法提供的检测限和定量限（LOD 和 LOQ）分别在 0.01-1.46mg/L 和 0.03-4.9mg/L 的范围内。与 DAD 相比，FLD 对黄烷醇、芪类和苯乙醇类似物的 LOD 和 LOQ 提高了 65%至 1000%。DAD 与 FLD 的结合提高了灵敏度和减少干扰信号的能力。所开发的方法已应用于葡萄酒、果穗茎和葡萄藤中 PCs 的特征描述。
