Grupo de Bioquímica Vegetal, Instituto de Biología Agrícola de Mendoza (IBAM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)-Universidad Nacional de Cuyo (UNCuyo), Almirante Brown 500, M5528AHB Chacras de Coria, Argentina.
Grupo de Bioquímica Vegetal, Instituto de Biología Agrícola de Mendoza (IBAM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)-Universidad Nacional de Cuyo (UNCuyo), Almirante Brown 500, M5528AHB Chacras de Coria, Argentina; Instituto Argentino de Veterinaria Ambiente y Salud (IAVAS), Universidad Juan A. Maza, Lateral Sur del Acceso, Este 2245, 5519 Guaymallén, Argentina.
Food Chem. 2023 Mar 30;405(Pt A):134718. doi: 10.1016/j.foodchem.2022.134718. Epub 2022 Oct 28.
This study aimed to determine the stability of PCs in grape canes extracts stored at different temperatures and light conditions. The PCs composition was monitored every-two weeks during three months by liquid chromatography coupled to diode array and fluorescence detectors (LC-DAD-FLD). Initially, stilbenes represented 87 % of total PCs. Storage at -20 and 5 °C reduced PCs 8 and 6 %, respectively. When extracts were exposed to 25 and 40 °C, the degradation of (+)-catechin and (-)-epicatechin was faster than under lower temperatures, and light accelerated the degradation kinetics. trans-piceatannol showed particularly sensitive to temperature increase, being mostly degraded after two weeks stored at 40 °C. Conversely, degradation of trans-resveratrol and ε-viniferin was mostly catalyzed by light, since nearly 70 % of them were degraded at 40 °C under light, in comparison with a 23 % reduction of trans-resveratrol and no changes of ε-viniferin at 40 °C in darkness.
本研究旨在确定储存在不同温度和光照条件下的葡萄藤提取物中原花青素(PCs)的稳定性。在三个月的时间里,每隔两周通过液相色谱-二极管阵列和荧光检测器(LC-DAD-FLD)监测 PCs 的组成。最初,白藜芦醇类物质占总 PCs 的 87%。在-20°C 和 5°C 下储存时,分别降低了 8%和 6%的 PCs。当提取物暴露于 25°C 和 40°C 时,(+)-儿茶素和(-)-表儿茶素的降解速度比低温下更快,而光照加速了降解动力学。反式白藜芦醇醇对温度升高特别敏感,在 40°C 下储存两周后,大部分被降解。相反,反式-白藜芦醇和 ε-viniferin 的降解主要由光照催化,因为在光照下,它们中的近 70%在 40°C 下被降解,而在黑暗中,反式-白藜芦醇的降解率为 23%,ε-viniferin 没有变化。