Orthodontics and Dentofacial Orthopedics, Eastman Institute for Oral Health, University of Rochester Medical Center, New York, USA.
School of Dental Sciences, Universiti Sains Malaysia, Health Campus, 16150, Kubang Kerian, Kelantan, Malaysia.
Prog Orthod. 2018 Apr 16;19(1):10. doi: 10.1186/s40510-018-0208-2.
Quality bone regeneration, which leads to the improvement of bone remodeling, is essential for orthodontic treatment. In order to improve bone regeneration and increase the amount of tooth movement, different techniques have been implemented. The object of this study is to compare the effects of low-level laser therapy (LLLT), low-intensity pulsed ultrasound (LIPUS), and their combination on bone remodeling during orthodontic tooth movement.
Eighty (80) male, 6-week-old Sprague Dawley rats were grouped in to four groups, the first group was irradiated with (940 nm) diode laser, second group with LIPUS, and third group with combination of both LLLT and LIPUS. A forth group used was a control group in an incomplete block split-mouth design. The LLLT and LIPUS were used to treat the area around the moving tooth once a day on days 0-7, then the experiment was ended in each experimental endpoint (1, 3, 7, 14, and 21 days). For amount of tooth movement, models were imaged and analyzed. Histological examination was performed after staining with (hematoxylin and eosin) and (alizarin red and Alcian Blue) stain. One step reverse transcription-polymerase chain reaction RT-PCR was also performed to elucidate the gene expression of RANK, RANKL, OPG, and RUNX-2.
The amount of tooth movement, the histological bone remodeling, and the RT-PCR were significantly greater in the treatment groups than that in the control group. Among the treatment groups, the combination group was the highest and the LIPUS group was the lowest.
These findings suggest that LLLT and LIPUS can enhance the velocity of tooth movement and improve the quality of bone remodeling during orthodontic tooth movement.
正畸治疗中，质量骨再生可改善骨重塑，这是必不可少的。为了改善骨再生并增加牙齿移动量，已经实施了不同的技术。本研究的目的是比较低水平激光治疗（LLLT）、低强度脉冲超声（LIPUS）及其组合在正畸牙齿移动过程中对骨重塑的影响。
将 80 只雄性、6 周龄 Sprague Dawley 大鼠分为 4 组，第一组用（940nm）二极管激光照射，第二组用 LIPUS，第三组用 LLLT 和 LIPUS 联合照射。第四组是不完全分块劈裂口设计的对照组。LLLT 和 LIPUS 用于在第 0-7 天每天治疗移动牙齿周围区域，然后在每个实验终点（1、3、7、14 和 21 天）结束实验。对于牙齿移动量，通过成像和分析模型进行评估。用（苏木精和伊红）和（茜素红和阿利新蓝）染色进行组织学检查。还进行了一步逆转录聚合酶链反应（RT-PCR），以阐明 RANK、RANKL、OPG 和 RUNX-2 的基因表达。
与对照组相比，治疗组的牙齿移动量、组织学骨重塑和 RT-PCR 均显著增加。在治疗组中，联合组最高，LIPUS 组最低。
这些发现表明，LLLT 和 LIPUS 可以加速牙齿移动速度，并改善正畸牙齿移动过程中的骨重塑质量。
