Kostryco Mikołaj, Chwil Mirosława, Matraszek-Gawron Renata
Department of Botany and Plant Physiology, University of Life Sciences in Lublin, Akademicka 15 Street, 20-950 Lublin, Poland.
Plants (Basel). 2020 Sep 12;9(9):1194. doi: 10.3390/plants9091194.
The genus is one of the largest taxonomically diverse and complex genera in the family Rosaceae. Morphology of pollen grains (equatorial and polar axes length, shape and size, aperture position, exine sculpture, perforations) is regarded as one of its main diagnostic features for identification of species and varieties. An attempt was made to fill the gap concerning the pollen micromorphology and ultrastructure of L. using light, scanning, and electron transmission microscopy. This study is a comparative analysis of micromorphological and ultrastructural traits of pollen from six raspberry cultivars. The pollen grains were classified as small or medium of shape . The parallel striae in the equatorial view in the exine sculpture were sometimes branched dichotomously in 'Glen Ample', 'Polka', and 'Polana', arcuate in 'Laszka' and 'Pokusa', or irregularly overlapping in 'Radziejowa'. The width of exine striae of biennial fruiting cultivars was much larger than in repeated fruiting cultivars. In terms of the increasing number of perforations per unit area of the exine surface, the cultivars were ranked as follows: 'Pokusa' < 'Glen Ample' < 'Laszka' < 'Polka' < 'Polana' < 'Radziejowa'. The thickest tectum, the highest and thickest columellae with the largest distances between them, and the thicker foot layer were demonstrated in 'Glen Ample'. The ectoexine constituted on average ca. 78-90% of the exine thickness. The findings may constitute auxiliary traits i.a. for identification of related taxa, interpretation of phylogenetic relationships, and pollination biology.
该属是蔷薇科中分类学上最多样化和最复杂的属之一。花粉粒的形态（赤道轴和极轴长度、形状和大小、萌发孔位置、外壁纹饰、穿孔）被视为鉴定物种和品种的主要诊断特征之一。本研究试图通过光学显微镜、扫描电子显微镜和透射电子显微镜填补有关L.花粉微观形态和超微结构方面的空白。本研究对六个树莓品种花粉的微观形态和超微结构特征进行了比较分析。花粉粒被分类为小型或中型。外壁纹饰在赤道面观的平行条纹在‘格伦安普尔’、‘波尔卡’和‘波拉纳’中有时二叉分枝，在‘拉斯卡’和‘波库萨’中呈弓形，在‘拉德齐约瓦’中不规则重叠。两年生结果品种的外壁条纹宽度比多次结果品种大得多。就外壁表面每单位面积穿孔数量的增加而言，各品种的排名如下：‘波库萨’<‘格伦安普尔’<‘拉斯卡’<‘波尔卡’<‘波拉纳’<‘拉德齐约瓦’。‘格伦安普尔’的覆盖层最厚，柱状层最高且最厚，它们之间的距离最大，足层也更厚。外壁平均约占外壁厚度的78 - 90%。这些发现可能构成辅助特征，用于相关分类群的鉴定、系统发育关系的解释以及传粉生物学研究等。
