Zhang Lihong, Lu Tao, Yang Ye, Hu Liangfeng
Department of Clinical Laboratory Center, Shaoxing People's Hospital, Shaoxing, Zhejiang 312000, P.R. China.
Department of Pathology, Shaoxing People's Hospital, Shaoxing, Zhejiang 312000, P.R. China.
Oncol Lett. 2020 Nov;20(5):152. doi: 10.3892/ol.2020.12003. Epub 2020 Aug 24.
The expression levels of α-enolase, also known as enolase 1 (ENO1), in liver cancer tissues and the autoantibody levels of ENO1 in the sera of patients with liver cancer were detected to investigate the function of ENO1 in the invasion and metastasis of liver cancer, as well as its clinical diagnostic value. Small interfering RNA (siRNA) was used to disrupt ENO1 gene expression in HepG2 and Huh7 liver cancer cells. The proliferation ability of liver cancer cells was assessed using Cell Counting Kit-8 (CCK-8); the migration ability of liver cancer cells was assessed using scratch tests; and the migration and invasion abilities of liver cancer cells were assessed using Transwell assays. ENO1 expression in liver cancer tissues (43.8%) was significantly higher than that in benign liver lesions (15.2%) (P=0.005). The serum anti-ENO1 antibody levels in the liver cancer group were significantly higher than those in the control and benign liver lesion groups (P<0.001). After ENO1 gene interference, the proliferation, migration and invasion abilities of HepG2 and Huh7 liver cancer cells exhibited different degrees of suppression. The results revealed that ENO1 promotes liver cancer invasion and metastasis; ENO1 plays an important role in liver cancer and can be used as a potential liver cancer-associated marker.
检测肝癌组织中α-烯醇化酶（也称为烯醇化酶1，ENO1）的表达水平以及肝癌患者血清中ENO1自身抗体水平，以研究ENO1在肝癌侵袭和转移中的作用及其临床诊断价值。使用小干扰RNA（siRNA）破坏HepG2和Huh7肝癌细胞中ENO1基因的表达。使用细胞计数试剂盒-8（CCK-8）评估肝癌细胞的增殖能力；使用划痕试验评估肝癌细胞的迁移能力；使用Transwell试验评估肝癌细胞的迁移和侵袭能力。肝癌组织中ENO1的表达（43.8%）显著高于良性肝病变组织（15.2%）（P=0.005）。肝癌组血清抗ENO1抗体水平显著高于对照组和良性肝病变组（P<0.001）。ENO1基因干扰后，HepG2和Huh7肝癌细胞的增殖、迁移和侵袭能力均受到不同程度的抑制。结果表明，ENO1促进肝癌侵袭和转移；ENO1在肝癌中起重要作用，可作为潜在的肝癌相关标志物。
