Life Sciences, Imperial College London, London, Greater London, SW7 2AZ, UK.
F1000Res. 2020 Aug 10;9:963. doi: 10.12688/f1000research.22464.1. eCollection 2020.
The availability of transparent zebrafish mutants (either : or ) for live imaging studies together with the ease of generating transgenic lines are two of the strengths of the zebrafish model organism. The fact that transparent ( and silver ( mutants are indistinguishable by eye at early stages (1-5 days post-fertilization; dpf) means many fish must be raised and later culled if they are not transparent. To identify translucent mutants early and easily at the early larval stage (≤5 dpf) before they are classified as protected animals, we developed a simple screening method using standard fluorescence microscopy. We estimate that this procedure could annually save 60,000 animals worldwide.
透明斑马鱼突变体（无论是 还是 ）的可用性，以及转基因系的易于生成，是斑马鱼模式生物的两个优势。透明 （ 和银色 （ 突变体在早期（受精后 1-5 天；dpf）阶段无法用眼区分这一事实意味着，如果它们不是透明的，许多鱼必须被饲养，然后再被淘汰。为了在早期幼虫阶段（≤5 dpf）之前尽早轻松地识别半透明突变体，在它们被归类为受保护动物之前，我们开发了一种使用标准荧光显微镜的简单筛选方法。我们估计，这一程序每年可以在全球范围内拯救 6 万只动物。
