Department of Interventional Therapy, The Second Affiliated Hospital of Wannan Medical College, Wuhu, China.
Department of Cardiology, Jizhou District People's Hospital, Tianjin, China.
Eur J Clin Microbiol Infect Dis. 2021 Mar;40(3):503-513. doi: 10.1007/s10096-020-04036-x. Epub 2020 Sep 16.
Breast cancer is one of the most common malignant tumors in women. More than half of breast cancer patients are not menopausal at the time of diagnosis. The occurrence and development of premenopausal breast cancer are affected by many factors. Intestinal flora, especially SCFA-producing bacteria, participates in the development of various tumors, and there is a lack of in-depth research in premenopausal breast cancer patients. We used 16S rRNA gene sequencing, targeted metabolomics, and cell culture methods to analyze the changes in the intestinal flora and metabolites of premenopausal breast cancer patients. In addition, we treated breast cancer cells with significantly altered propionate and butyrate in vitro to examine their effects on cell activity. This study followed STROBE guidelines. We found that compared with healthy premenopausal women, the composition and symbiosis of intestinal flora in patients with premenopausal breast cancer changed significantly. The abundance of short-chain fatty acid (SCFA)-producing bacteria was significantly reduced, and the key SCFA-producing enzymes were also significantly reduced. Pediococcus and Desulfovibrio could distinguish premenopausal breast cancer patients from normal premenopausal women. The related propionate and butyrate had a certain ability to inhibit breast cancer cell viability in vitro. As SCFA-producing bacteria, Pediococcus and Desulfovibrio showed potential reference value for the diagnosis of premenopausal breast cancer. The ability of propionate and butyrate to inhibit breast cancer cell lines in vitro suggests that the relevant SCFA receptor may be a new target for the treatment of premenopausal breast cancer.
乳腺癌是女性最常见的恶性肿瘤之一。超过一半的乳腺癌患者在诊断时未绝经。绝经前乳腺癌的发生和发展受多种因素影响。肠道菌群，尤其是产生 SCFA 的细菌，参与了各种肿瘤的发生发展，而绝经前乳腺癌患者的相关研究还不够深入。我们采用 16S rRNA 基因测序、靶向代谢组学和细胞培养方法，分析了绝经前乳腺癌患者肠道菌群和代谢物的变化。此外，我们还在体外用明显改变的丙酸盐和丁酸盐处理乳腺癌细胞，以研究它们对细胞活性的影响。本研究遵循 STROBE 指南。我们发现，与健康的绝经前女性相比，绝经前乳腺癌患者的肠道菌群组成和共生关系发生了显著变化。产短链脂肪酸（SCFA）细菌的丰度显著降低，关键的 SCFA 产生酶也显著降低。肠球菌和脱硫弧菌可以将绝经前乳腺癌患者与正常绝经前女性区分开来。相关的丙酸盐和丁酸盐在体外具有一定的抑制乳腺癌细胞活力的能力。作为产 SCFA 的细菌，肠球菌和脱硫弧菌对绝经前乳腺癌的诊断具有潜在的参考价值。丙酸盐和丁酸盐在体外抑制乳腺癌细胞系的能力表明，相关的 SCFA 受体可能成为绝经前乳腺癌治疗的新靶点。
