Guangxi Key Laboratory of Marine Environmental Science, Beibu Gulf Marine Research Center, Guangxi Academy of Sciences, Nanning 530007, PR China.
Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry, Beibu Gulf Marine Research Center, Guangxi Academy of Sciences, Nanning 530007, PR China.
Int J Syst Evol Microbiol. 2021 Feb;71(2). doi: 10.1099/ijsem.0.004624.
A Gram-stain-negative, aerobic, mesophilic, non-motile bacterium, designated M0104, was isolated from a gorgonian coral collected from Xieyang island, Guangxi Province, PR China. Colonies of the strain were non-motile cocci and pink. The strain grew at 15-34 °C (optimum, 28 °C), pH 4.5-8.0 (optimum, pH 7.0) and with 0-4% (w/v) NaCl (optimum, 0-2 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain M0104 was closely related to JCM 31275 (96.2 %), KCTC 62043 (96.0 %), JCM 31878 (95.9 %) and KCTC 42542 (95.7 %). The strain had an assembly size of 5.0 Mb and a G+C content of 71.0mol%. Genes involved in copper, cadmium, lead, arsenic and zinc resistance were identified in the genome of strain M0104. The digital DNA-DNA hybridization and average nucleotide identity values between the genome sequence of strain M0104 and those of closely related type strains were 19.4-24.9 % and 74.3-81.8 %, respectively. Strain M0104 contained C 7, C 3, anteiso C and C as the major fatty acids (>7 %) and ubiquinone Q-10 as the sole isoprenoid quinone. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine were its major polar lipids. Based on its phenotypic, phylogenetic and chemotaxonomic properties, strain M0104 is proposed to represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is M0104 (=KCTC 62359=MCCC 1K03632).
一株革兰氏阴性、需氧、中温、非运动的细菌，被命名为 M0104，从中国广西省斜阳岛采集的柳珊瑚中分离得到。该菌株的菌落为非运动性球菌，呈粉红色。该菌株在 15-34°C（最佳温度为 28°C）、pH4.5-8.0（最佳 pH 值为 7.0）和 0-4%（w/v）NaCl（最佳为 0-2%）的条件下生长。基于 16S rRNA 基因序列的系统发育分析表明，菌株 M0104与 JCM 31275（96.2%）、KCTC 62043（96.0%）、JCM 31878（95.9%）和 KCTC 42542（95.7%）密切相关。该菌株的基因组大小为 5.0Mb，G+C 含量为 71.0mol%。在菌株 M0104 的基因组中鉴定出与铜、镉、铅、砷和锌抗性相关的基因。菌株 M0104 的基因组与密切相关的模式菌株的数字 DNA-DNA 杂交和平均核苷酸同一性值分别为 19.4-24.9%和 74.3-81.8%。菌株 M0104 含有 C7、C3、anteiso C 和 C 作为主要脂肪酸（>7%），并以泛醌 Q-10 作为唯一的异戊二烯醌。二磷酸甘油酯、磷脂酰乙醇胺、磷脂酰甘油和磷脂酰胆碱是其主要的极性脂。基于其表型、系统发育和化学分类学特性，菌株 M0104 代表一个新的种属，属于 属，建议命名为 sp. nov.。其模式菌株为 M0104（=KCTC 62359=MCCC 1K03632）。
