sp. nov., a heavy metal resistant bacterium isolated from coral.

A Gram-stain-negative, aerobic, mesophilic, non-motile bacterium, designated M0104, was isolated from a gorgonian coral collected from Xieyang island, Guangxi Province, PR China. Colonies of the strain were non-motile cocci and pink. The strain grew at 15-34 °C (optimum, 28 °C), pH 4.5-8.0 (optimum, pH 7.0) and with 0-4% (w/v) NaCl (optimum, 0-2 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain M0104 was closely related to JCM 31275 (96.2 %), KCTC 62043 (96.0 %), JCM 31878 (95.9 %) and KCTC 42542 (95.7 %). The strain had an assembly size of 5.0 Mb and a G+C content of 71.0mol%. Genes involved in copper, cadmium, lead, arsenic and zinc resistance were identified in the genome of strain M0104. The digital DNA-DNA hybridization and average nucleotide identity values between the genome sequence of strain M0104 and those of closely related type strains were 19.4-24.9 % and 74.3-81.8 %, respectively. Strain M0104 contained C 7, C 3, anteiso C and C as the major fatty acids (>7 %) and ubiquinone Q-10 as the sole isoprenoid quinone. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine were its major polar lipids. Based on its phenotypic, phylogenetic and chemotaxonomic properties, strain M0104 is proposed to represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is M0104 (=KCTC 62359=MCCC 1K03632).