ETEP (Etiology and Therapy of Periodontal and Peri-implant Diseases) Research Group, University Complutense of Madrid, 28040 Madrid, Spain.
Medicine Department, Faculty of Medicine, University Complutense of Madrid, 28040 Madrid, Spain.
Nutrients. 2020 Sep 14;12(9):2812. doi: 10.3390/nu12092812.
In search for natural products with antimicrobial properties for use in the prevention and treatment of periodontitis, the purpose of this investigation was to evaluate the antimicrobial activity of two omega-3 fatty acids, docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), using an in vitro multi-species subgingival biofilm model including , , , , , and . The antimicrobial activities of EPA and DHA extracts (100 µM) and the respective controls were assessed on 72 h biofilms by their submersion onto discs for 60 s. Antimicrobial activity was evaluated by quantitative polymerase chain reaction (qPCR), confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). ANOVA with Bonferroni correction was used to evaluate the antimicrobial activity of each of the fatty acids. Both DHA and EPA significantly reduced ( < 0.001 in all cases) the bacterial strains used in this biofilm model. The results with CLSM were consistent with those reported with qPCR. Structural damage was evidenced by SEM in some of the observed bacteria. It was concluded that both DHA and EPA have significant antimicrobial activity against the six bacterial species included in this biofilm model.
在寻找具有抗菌特性的天然产物以用于预防和治疗牙周炎的过程中，本研究旨在评估两种 ω-3 脂肪酸，即二十二碳六烯酸（DHA）和二十碳五烯酸（EPA），使用包括 、 、 、 、 和 在内的体外多物种龈下生物膜模型，评估 EPA 和 DHA 提取物（100 µM）及其相应对照物在 72 h 生物膜中的抗菌活性，通过将其浸入到载玻片上 60 s 来进行。抗菌活性通过定量聚合酶链反应（qPCR）、共聚焦激光扫描显微镜（CLSM）和扫描电子显微镜（SEM）进行评估。采用方差分析（ANOVA）和 Bonferroni 校正来评估每种脂肪酸的抗菌活性。DHA 和 EPA 均显著减少（在所有情况下均 < 0.001）了本生物膜模型中使用的细菌菌株。CLSM 的结果与 qPCR 的结果一致。SEM 观察到一些细菌出现结构损伤。结论是，DHA 和 EPA 对本生物膜模型中包含的六种细菌均具有显著的抗菌活性。
