证明黄烷间键合和原花青素的没食子酸化在维持长期牙本质生物改性中的作用。
Evidence to the role of interflavan linkages and galloylation of proanthocyanidins at sustaining long-term dentin biomodification.
机构信息
Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA.
Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, Chicago, IL, USA.
出版信息
Dent Mater. 2019 Feb;35(2):328-334. doi: 10.1016/j.dental.2018.11.029. Epub 2018 Dec 21.
OBJECTIVES
The interactivity of proanthocyanidins (PACs) with collagen modulates dentin matrix biomechanics and biostability. Herein, PAC extracts selected based on structural diversity were investigated to determine key PAC features driving sustained effects on dentin matrices over a period of 18months.
METHODS
The chemical profiles of PAC-rich plant sources, Pinus massoniana (PM), Cinnamomum verum (CV) and Hamamelis virginiana (HV) barks, as well as Vitis vinifera (VV) seeds, were obtained by diol HPLC analysis after partitioning of the extracts between methyl acetate and water. Dentin matrices (n=15) were prepared from human molars to determine the apparent modulus of elasticity over 18months of aging. Susceptibility of the dentin matrix to degradation by endogenous and exogenous proteases was determined by presence of solubilized collagen in supernatant, and resistance to degradation by bacterial collagenase, respectively. Data were analyzed using ANOVA and Games-Howell post hoc tests (α=0.05).
RESULTS
After 18months, dentin matrices modified by PM and CV extracts, containing only non-galloylated PACs, were highly stable mechanically (p<0.05). Dentin matrices treated with CV exhibited the lowest degradation by bacterial collagenase after 1h and 18months of aging (p<0.05), while dentin matrices treated with PM showed the least mass loss and collagen solubilization by endogenous enzymes over time (p<0.05).
SIGNIFICANCE
Resistance against long-term degradation was observed for all experimental groups; however, the most potent and long-lasting dentin biomodification resulted from non-galloylated PACs.
目的
原花青素（PACs）与胶原蛋白的相互作用调节牙本质基质的生物力学和生物稳定性。在此，根据结构多样性选择的 PAC 提取物被研究，以确定在 18 个月的时间内对牙本质基质持续产生影响的关键 PAC 特征。
方法
采用二醇 HPLC 分析，从马尾松（PM）、肉桂（CV）和金缕梅（HV）树皮以及葡萄（VV）种子等富含 PAC 的植物源提取物中获得化学成分谱，将提取物分配在乙酸甲酯和水之间。从人磨牙中制备牙本质基质（n=15），以确定在 18 个月老化过程中的弹性模量。通过上清液中可溶性胶原蛋白的存在来确定牙本质基质对内源和外源蛋白酶降解的敏感性，以及抵抗细菌胶原酶降解的能力。采用方差分析和 Games-Howell 事后检验（α=0.05）进行数据分析。
结果
18 个月后，仅含有非没食子酰化 PAC 的 PM 和 CV 提取物改性的牙本质基质在机械稳定性方面表现出色（p<0.05）。经 CV 处理的牙本质基质在 1h 和 18 个月老化后表现出最低的细菌胶原酶降解（p<0.05），而经 PM 处理的牙本质基质在较长时间内显示出最低的质量损失和内源性酶引起的胶原蛋白溶解（p<0.05）。
意义
所有实验组均观察到对长期降解的抵抗力；然而，最有效和持久的牙本质生物改性来自于非没食子酰化 PAC。
Zotero 插件