Department of Restorative Dentistry, Araçatuba Dental School, Univ. Estadual Paulista, Araçatuba, SP, Brazil.
Department of Dentistry, Endodontic and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
J Appl Oral Sci. 2015 May-Jun;23(3):302-9. doi: 10.1590/1678-775720140304.
Dental caries is considered a disease of high prevalence and a constant problem in public health. Proanthocyanidins (PAs) are substances that have been the target of recent studies aiming to control or treat caries.
The aim of this in vitro study was to evaluate the efficacy of a treatment with grape seed extract, under cariogenic challenge, to minimize or even prevent the onset of caries in the enamel and dentin.
Blocks of enamel and dentin (6.0x6.0 mm) were obtained from bovine central incisors, polished, and selected by analysis of surface microhardness (SH). The blocks were randomly divided into 3 groups (n=15), according to the following treatments: GC (control), GSE (grape seed extract), GF (fluoride - 1,000 ppm). The blocks were subjected to 6 daily pH cycles for 8 days. Within the daily cycling, the specimens were stored in buffered solution. The blocks were then analyzed for perpendicular and surface hardness and polarized light microscopy.
The means were subjected to statistical analysis using the ANOVA and Fisher's PLSD tests (p<0.05). For enamel SH, GF showed the highest hardness values. In the dentin, GF was also the one that showed higher hardness values, followed by GSE. Regarding the cross-sectional hardness values, all groups behaved similarly in both the enamel and dentin. The samples that were treated with GSE and fluoride (GF) showed statistically higher values than the control.
Based on the data obtained in this in vitro study, it is suggested that grape seed extract inhibits demineralization of artificial carious lesions in both the enamel and dentin, but in a different scale in each structure and in a smaller scale when compared to fluoride.
本体外研究旨在评估葡萄籽提取物在致龋挑战下的治疗效果，以最小化甚至预防釉质和牙本质龋的发生。
从牛侧切牙获得釉质和牙本质（6.0x6.0mm）块，抛光并通过表面显微硬度（SH）分析进行选择。块随机分为 3 组（n=15），根据以下处理：GC（对照）、GSE（葡萄籽提取物）、GF（氟化物-1000ppm）。块经受 6 天每天 pH 循环 8 天。在每日循环中，将标本保存在缓冲溶液中。然后对块进行垂直和表面硬度以及偏光显微镜分析。
根据本体外研究获得的数据，建议葡萄籽提取物抑制人工龋损在釉质和牙本质中的脱矿作用，但在每种结构中的作用程度不同，与氟化物相比作用程度较小。
