Lander E S, Waterman M S
Whitehead Institute for Biomedical Research, Cambridge Center, Massachusetts 02142.
Genomics. 1988 Apr;2(3):231-9. doi: 10.1016/0888-7543(88)90007-9.
Results from physical mapping projects have recently been reported for the genomes of Escherichia coli, Saccharomyces cerevisiae, and Caenorhabditis elegans, and similar projects are currently being planned for other organisms. In such projects, the physical map is assembled by first "fingerprinting" a large number of clones chosen at random from a recombinant library and then inferring overlaps between clones with sufficiently similar fingerprints. Although the basic approach is the same, there are many possible choices for the fingerprint used to characterize the clones and the rules for declaring overlap. In this paper, we derive simple formulas showing how the progress of a physical mapping project is affected by the nature of the fingerprinting scheme. Using these formulas, we discuss the analytic considerations involved in selecting an appropriate fingerprinting scheme for a particular project.
最近已有关于大肠杆菌、酿酒酵母和秀丽隐杆线虫基因组物理图谱绘制项目的结果报道，目前也正在为其他生物体规划类似项目。在这些项目中，物理图谱的构建首先是对从重组文库中随机选取的大量克隆进行“指纹识别”，然后推断具有足够相似指纹的克隆之间的重叠情况。尽管基本方法相同，但用于表征克隆的指纹以及声明重叠的规则有许多可能的选择。在本文中，我们推导出简单公式，展示了物理图谱绘制项目的进展如何受到指纹识别方案性质的影响。利用这些公式，我们讨论了为特定项目选择合适的指纹识别方案时涉及的分析考量。
