School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, People's Republic of China.
ACS Appl Mater Interfaces. 2020 Oct 14;12(41):46539-46548. doi: 10.1021/acsami.0c12593. Epub 2020 Sep 29.
Nanozymes as one of artificial enzymes show many advantages than natural enzymes. The high Michaelis-Menten constant () to HO is the drawback for nanozymes, which means a high HO concentration to oxidize 3,3',5,5'-tetramethylbenzidine (TMB). For this problem, FeS/SiO double mesoporous hollow spheres (DMHSs) were first synthesized as an artificial peroxidase through a solid reaction. The experimental results demonstrate that FeO vulcanization and DMHS formation were effective strategies to enhance affinity to HO for the nanozyme. The of FeS/SiO DMHSs (HO as the substrate) is 18-fold smaller than that of FeS nanoparticles (NPs). The catalytic efficiency (/) of FeS/SiO DMHSs is about 16 times higher than that of FeS NPs. FeS/SiO DMHSs can be used as a nanozyme to sensitively and rapidly detect HO and glutathione within 1 min at room temperature.
纳米酶作为一种人工酶，比天然酶具有许多优势。高米氏常数（）对 HO 是纳米酶的一个缺点，这意味着需要高浓度的 HO 来氧化 3,3',5,5'-四甲基联苯胺（TMB）。针对这个问题，首次通过固相反应合成了 FeS/SiO 双介孔空心球（DMHSs）作为人工过氧化物酶。实验结果表明，FeO 硫化和 DMHS 的形成是增强纳米酶对 HO 亲和力的有效策略。FeS/SiO DMHSs（以 HO 为底物）的（HO）为 18 倍，低于 FeS 纳米颗粒（NPs）。FeS/SiO DMHSs 的催化效率（/）比 FeS NPs 高约 16 倍。FeS/SiO DMHSs 可作为纳米酶，在室温下 1 分钟内灵敏快速地检测 HO 和谷胱甘肽。
