Sorbonne Université, Institut du Cerveau (ICM), Campus Hospitalier Universitaire Pitié-Salpêtrière, 47 bld de l'Hôpital, 75013, Paris, France.
Muséum National d'Histoire Naturelle (MNHN), CNRS UMR 7221, Paris, France.
Sci Rep. 2020 Sep 17;10(1):15235. doi: 10.1038/s41598-020-72039-x.
Pharmacological experiments indicate that neuropeptides can effectively tune neuronal activity and modulate locomotor output patterns. However, their functions in shaping innate locomotion often remain elusive. For example, somatostatin has been previously shown to induce locomotion when injected in the brain ventricles but to inhibit fictive locomotion when bath-applied in the spinal cord in vitro. Here, we investigated the role of somatostatin in innate locomotion through a genetic approach by knocking out somatostatin 1.1 (sst1.1) in zebrafish. We automated and carefully analyzed the kinematics of locomotion over a hundred of thousand bouts from hundreds of mutant and control sibling larvae. We found that the deletion of sst1.1 did not impact acousto-vestibular escape responses but led to abnormal exploration. sst1.1 mutant larvae swam over larger distance, at higher speed and performed larger tail bends, indicating that Somatostatin 1.1 inhibits spontaneous locomotion. Altogether our study demonstrates that Somatostatin 1.1 innately contributes to slowing down spontaneous locomotion.
药理学实验表明，神经肽可以有效地调节神经元活动，并调节运动输出模式。然而，它们在塑造先天运动中的作用常常难以捉摸。例如，先前的研究表明，生长抑素注射到脑室中会引起运动，而在体外脊髓灌流时会抑制 fictive 运动。在这里，我们通过基因敲除斑马鱼中的生长抑素 1.1 (sst1.1)，通过遗传方法研究生长抑素在先天运动中的作用。我们自动化并仔细分析了来自数百个突变体和对照同胞幼虫的数十万次运动的运动学。我们发现，sst1.1 的缺失并不影响声-前庭逃避反应，但导致异常探索。sst1.1 突变体幼虫游动的距离更大、速度更快，尾巴弯曲幅度更大，表明生长抑素 1.1 抑制了自发性运动。总的来说，我们的研究表明生长抑素 1.1 先天有助于减缓自发性运动。
