Flores Daniel R, Casados Luz E, Velasco Sandra F, Ramírez Ana C, Velázquez Gilberto
Chemistry Department, University of Guadalajara, 1421 Marcelino García Barragán Blvd, 44430 Guadalajara, Jalisco Mexico.
Life and Science Division, Food Department, University of Guanajuato, Campus Irapuato-Salamanca, Km 9 carretera Irapuato-Silao ap 311, 36500 Irapuato, Guanajuato Mexico.
BMC Chem. 2020 Sep 9;14(1):55. doi: 10.1186/s13065-020-00707-0. eCollection 2020 Dec.
In the case of Tenebrionidae family insects, studies focus on larval stage, leaving a lack of information regarding other stages. Therefore, this study was performed in order to understand the differences between the nutritional composition and the bioactivity of two species of this family in their adult stage, fed with a specific diet. Adult beetles of both species were defatted, lyophilized and protein extracted with buffer. Proximal and phytochemical analysis of the extracts of each insect were performed, along with protein extract and hydrolysis analysis by Tris-Tricine and Tris Glycine SDS PAGE. This analysis showed that contained more protein and fat than but contained less crude fiber. The protein extraction was made with PBS, where 130 and 45 kDa bands showed predominant for and less protein was present for . Antioxidant and antimicrobial activities of the enzymatic protein hydrolysates and protein crude extracts were determined. Presence of protein associated with the antioxidant activity were found in both insects. Nonetheless had a higher antioxidant activity with the protein extract in contrast with the higher antioxidant activity shown by once the extracts were digested. After proteolysis, protein extracts showed an increasing antioxidant activity, plus, the ability to inhibit microbial growth of , and . Insect protein hydrolysates with protease open the possibility for the use of these beetles as new sources of encrypted peptides for microbiological control once characterized.
对于拟步甲科昆虫，研究主要集中在幼虫阶段，而关于其他阶段的信息较少。因此，本研究旨在了解该科两种昆虫成虫阶段在特定饮食喂养下营养成分和生物活性的差异。两种昆虫的成虫均进行脱脂、冻干处理，并用缓冲液提取蛋白质。对每种昆虫提取物进行了近似成分和植物化学分析，以及通过Tris - Tricine和Tris甘氨酸SDS - PAGE进行蛋白质提取物和水解分析。该分析表明，[昆虫名称1]比[昆虫名称2]含有更多的蛋白质和脂肪，但粗纤维含量较少。蛋白质提取采用PBS，其中130 kDa和45 kDa条带在[昆虫名称1]中占主导，而[昆虫名称2]中蛋白质含量较少。测定了酶解蛋白水解产物和蛋白质粗提物的抗氧化和抗菌活性。两种昆虫中均发现了与抗氧化活性相关的蛋白质。然而，[昆虫名称1]的蛋白质提取物具有较高的抗氧化活性，而[昆虫名称2]在提取物消化后表现出较高的抗氧化活性。蛋白水解后，蛋白质提取物的抗氧化活性增强，此外，还具有抑制[微生物名称1]、[微生物名称2]和[微生物名称3]生长的能力。一旦对昆虫蛋白水解产物用蛋白酶进行表征，就有可能将这些甲虫用作微生物控制中新型加密肽的来源。
