Department of Chemistry 'Giacomo Ciamician', University of Bologna, Via Selmi 2, Bologna, Italy.
Interdepartmental Centre for Renewable Sources, Environment, Sea and Energy (CIRI FRAME), Alma Mater Studiorum - University of Bologna, Via Sant'Alberto 163, Ravenna, Italy.
Luminescence. 2021 Mar;36(2):278-293. doi: 10.1002/bio.3948. Epub 2020 Oct 14.
Recent advancements in synthetic biology, organic chemistry, and computational models have allowed the application of bioluminescence in several fields, ranging from well established methods for detecting microbial contamination to in vivo imaging to track cancer and stem cells, from cell-based assays to optogenetics. Moreover, thanks to recent technological progress in miniaturized and sensitive light detectors, such as photodiodes and imaging sensors, it is possible to implement laboratory-based assays, such as cell-based and enzymatic assays, into portable analytical devices for point-of-care and on-site applications. This review highlights some recent advances in the development of whole-cell and cell-free bioluminescence biosensors with a glance on current challenges and different strategies that have been used to turn bioassays into biosensors with the required analytical performance. Critical issues and unsolved technical problems are also highlighted, to give the reader a taste of this fascinating and challenging field.
近年来，合成生物学、有机化学和计算模型的进步使得生物发光在多个领域得到了应用，从用于检测微生物污染的成熟方法到体内成像以跟踪癌症和干细胞，从基于细胞的测定法到光遗传学。此外，由于最近在微型化和灵敏的光探测器方面取得的技术进步，例如光电二极管和成像传感器，有可能将基于实验室的测定法，例如基于细胞的和酶的测定法，集成到便携式分析设备中，用于即时和现场应用。本综述重点介绍了全细胞和无细胞生物发光生物传感器的最新进展，并简要介绍了当前的挑战以及为了将生物测定法转化为具有所需分析性能的生物传感器而采用的不同策略。还突出了关键问题和未解决的技术问题，让读者了解这个引人入胜且具有挑战性的领域。
