Department of Plant and Environmental Sciences, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel.
Research Center for Applied Sciences, Academia Sinica, Taipei 11529, Taiwan; Institute of Biophotonics, National Yang Ming Chao Tung University, Taipei 112304, Taiwan; Department of Mechanical and Mechatronic Engineering, National Taiwan Ocean University, Keelung 202301, Taiwan; College of Engineering, Chang Gung University, Taoyuan 33302, Taiwan.
Curr Opin Biotechnol. 2023 Aug;82:102952. doi: 10.1016/j.copbio.2023.102952. Epub 2023 May 30.
Relying on the biological responses and activity of living cells, bioluminescent whole-cell biosensors generate an optical signal in response to the presence of target compounds. The miniaturization of low-light detectors and their integration with microfluidics have allowed the realization of portable devices for sensitive imaging and quantification of these signals. This review thus focuses on bioluminescence-based whole-cell biosensors, integrated with handheld optical detectors, with an emphasis on the use of the low-light imaging capability of modern smartphones. We highlight state-of-the-art miniaturization techniques applied to the incorporation of living cells into diverse hardware platforms, and to the sensitive recording of the biologically generated photons. The integration of sample introduction, host-cell activity maintenance, luminescence acquisition, data processing/presentation, and portability are also discussed.
依赖于活细胞的生物反应和活性，生物发光全细胞生物传感器在存在目标化合物时会产生光学信号。微光探测器的小型化及其与微流控技术的集成，使得能够实现用于这些信号的敏感成像和定量的便携式设备。因此，本综述重点介绍了与手持式光学探测器集成的基于生物发光的全细胞生物传感器，重点介绍了现代智能手机的低光成像能力的应用。我们强调了应用于将活细胞整合到各种硬件平台中以及对生物产生的光子进行灵敏记录的最新小型化技术。还讨论了样品引入、宿主细胞活性维持、发光采集、数据处理/呈现和便携性的集成。
