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由苹果酒乳酸菌产生的胞外多糖制成的压缩模塑大豆蛋白薄膜。

Compression Molded Soy Protein Films with Exopolysaccharides Produced by Cider Lactic Acid Bacteria.

作者信息

Uranga Jone, Llamas Mª Goretti, Agirrezabala Ziortza, Dueñas María Teresa, Etxebeste Oier, Guerrero Pedro, de la Caba Koro

机构信息

BIOMAT Research Group, University of the Basque Country (UPV/EHU), Escuela de Ingeniería de Gipuzkoa, Plaza de Europa 1, 20018 Donostia-San Sebastián, Spain.

GLYCOBAL Research Group, Facultad de Química, University of the Basque Country (UPV/EHU), Paseo Manuel de Lardizabal 3, 20018 Donostia-San Sebastián, Spain.

出版信息

Polymers (Basel). 2020 Sep 16;12(9):2106. doi: 10.3390/polym12092106.

DOI:10.3390/polym12092106
PMID:32947835
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7570117/
Abstract

Two exopolysaccharide (EPS)-producing lactic acid bacteria (LAB) strains, CUPV281 and CUPV271, were isolated from Spanish apple must. Each of the strains produced a dextran, with different branching degrees, to be incorporated into soy protein isolate (SPI) film-forming formulations. Films were prepared by compression molding, a more rapid processing method than solution casting and, thus, with a greater potential for scaling-up production. Thermal analysis showed that SPI and EPS start the degradation process at temperatures above 190 °C, confirming that the compression temperature selected (120 °C) was well below the corresponding degradation temperatures. Resulting films were transparent and homogeneous, as shown by UV-Vis spectroscopy and SEM, indicating the good compatibility between SPI and EPS. Furthermore, FTIR analysis showed that the interactions between SPI and EPS were physical interactions, probably by hydrogen bonding among the polar groups of SPI and EPS. Regarding antifungal/fungistatic activity, LAB strains used in this study showed an inhibitory effect on germination of fungal spores.

摘要

从西班牙苹果汁中分离出两株产胞外多糖（EPS）的乳酸菌（LAB）菌株，即CUPV281和CUPV271。每株菌株都产生了具有不同分支程度的葡聚糖，用于掺入大豆分离蛋白（SPI）成膜配方中。通过压缩成型制备薄膜，这是一种比溶液浇铸更快的加工方法，因此具有更大的扩大生产潜力。热分析表明，SPI和EPS在高于190°C的温度下开始降解过程，证实所选的压缩温度（120°C）远低于相应的降解温度。如紫外可见光谱和扫描电子显微镜所示，所得薄膜是透明且均匀的，表明SPI和EPS之间具有良好的相容性。此外，傅里叶变换红外光谱分析表明，SPI和EPS之间的相互作用是物理相互作用，可能是通过SPI和EPS极性基团之间的氢键作用。关于抗真菌/抑菌活性，本研究中使用的LAB菌株对真菌孢子的萌发具有抑制作用。

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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e7e/7570117/6f6f8c5a7371/polymers-12-02106-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e7e/7570117/f9403f02b6d7/polymers-12-02106-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e7e/7570117/d0862b1a1239/polymers-12-02106-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e7e/7570117/4e18b0f425ed/polymers-12-02106-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e7e/7570117/202074ecb414/polymers-12-02106-g009.jpg
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