Chatziplis Dimitrios, Oikonomou Stavroula, Loukovitis Dimitrios, Tsiokos Dimitrios, Samaras Athanasios, Dimitroglou Arkadios, Kottaras Lefteris, Papanna Kantham, Papaharisis Leonidas, Tsigenopoulos Costas, Pavlidis Michail
Laboratory of Agrobiotechnology and Inspection of Agricultural Products, Dept of Agricultural Technology, School of Geotechnical Sciences, International Hellenic University, Alexander Campus, P.O. Box 141, 57 400 Sindos, Thessaloniki, Greece.
Department of Genetics, Development and Molecular Biology, Aristotle University of Thessaloniki, University Campus, 54124 Thessaloniki, Greece.
Animals (Basel). 2020 Sep 16;10(9):1668. doi: 10.3390/ani10091668.
There is a growing interest in selective breeding in European sea bass (), especially regarding family selection based on growth performance. In particular, quantitative trait loci (QTL) identification in sea bass enhances the application of marker-assisted breeding for the genetic improvement of the production traits. The aims of the study were to identify potential QTL affecting stress and immunological indicators, body weight, and mortality after vibriosis injection in sea bass as well as to estimate heritability and genetic/phenotypic correlations for the aforementioned traits. To this end, stress test was performed on 960 offspring and a sub-group of them (420) was selected to explore the mortality after vibrio injection. Selective genotyping was performed in 620 offspring for 35 microsatellite markers and distributed into 6 linkage groups. The length of the genetic linkage map was 283.6 cM and the mean distance between the markers was 8.1 cM. QTL affecting body weight in three different growth periods detected on linkage groups LG1, LG4, LG6, and LG14. A QTL associated with weight in early growth stages (290-306 days post-hatching) was also identified on LG3. QTL analysis confirmed the existence of QTL affecting cortisol levels, on LG3 and LG14. Moreover, new QTL affecting only cortisol and glucose levels were detected on LG1 and LG23. No QTL affecting hormonal or biochemical marks was found on LG4 and LG6. Heritability of cortisol, lysozyme levels, and mortality were high (0.36, 0.55, and 0.38, respectively).
欧洲海鲈的选择性育种越来越受到关注，尤其是基于生长性能的家系选择。特别是，海鲈数量性状基因座（QTL）的鉴定增强了标记辅助育种在生产性状遗传改良中的应用。本研究的目的是鉴定影响海鲈应激和免疫指标、体重以及弧菌注射后死亡率的潜在QTL，并估计上述性状的遗传力和遗传/表型相关性。为此，对960只后代进行了应激测试，并选择其中一个亚组（420只）来探究弧菌注射后的死亡率。对620只后代进行了35个微卫星标记的选择性基因分型，并将其分配到6个连锁群中。遗传连锁图谱的长度为283.6厘摩，标记之间的平均距离为8.1厘摩。在连锁群LG1、LG4、LG6和LG14上检测到影响三个不同生长时期体重的QTL。在LG3上也鉴定出一个与早期生长阶段（孵化后290 - 306天）体重相关的QTL。QTL分析证实了在LG3和LG14上存在影响皮质醇水平的QTL。此外，在LG1和LG23上检测到仅影响皮质醇和葡萄糖水平的新QTL。在LG4和LG6上未发现影响激素或生化指标的QTL。皮质醇、溶菌酶水平和死亡率的遗传力较高（分别为0.36、0.55和0.38）。
