Oikonomou Stavroula, Samaras Athanasios, Tekeoglou Maria, Loukovitis Dimitrios, Dimitroglou Arkadios, Kottaras Lefteris, Papanna Kantham, Papaharisis Leonidas, Tsigenopoulos Costas S, Pavlidis Michail, Chatziplis Dimitrios
Laboratory of Agrobiotechnology and Inspection of Agricultural Products, Department of Agricultural Technology, School of Geotechnical Sciences, International Hellenic University, Alexander Campus, P.O. Box 141, Sindos, 57 400 Thessaloniki, Greece.
Department of Genetics, Development and Molecular Biology, Aristotle University of Thessaloniki, University Campus, 54124 Thessaloniki, Greece.
Animals (Basel). 2022 Jan 23;12(3):277. doi: 10.3390/ani12030277.
The majority of the genetic studies in aquaculture breeding programs focus on commercial traits such as body weight, morphology, and resistance against diseases. However, studying stress response in European seabass may contribute to the understanding of the genetic component of stress and its future use to select broodstock whose offspring may potentially be less affected by handling. A total of 865 European seabass offspring were used to measure body weight and stress response. Moreover, a disease challenge experiment with was conducted in a subset (332) of the above fish to study disease resistance. Fish were genotyped with a 57k SNP array, and a Genome-Wide Association study (GWAS) was performed. Five SNPs were found to be statistically significant, three of which affect stress indicators and body weight (in a subgroup of the population), and a putative SNP affects growth performance, while no SNP associated with resistance to was found. A moderate to high genomic heritability regarding stress indicators and body weight was estimated using the Restricted Maximum Likelihood (REML) process. Finally, the accuracy, along with the correlation between Estimated Breeding Values (EBVs) and Genomic Estimated Breeding Values (GEBVs), were calculated for all the traits.
水产养殖育种计划中的大多数基因研究都集中在商业性状上，如体重、形态以及抗病能力。然而，对欧洲鲈鱼应激反应的研究可能有助于理解应激的遗传成分，以及未来用于选择亲鱼，其后代可能受到处理的影响较小。总共865尾欧洲鲈鱼后代被用于测量体重和应激反应。此外，对上述鱼的一个子集（332尾）进行了疾病挑战实验，以研究抗病性。用一个57k SNP阵列对鱼进行基因分型，并进行了全基因组关联研究（GWAS）。发现五个SNP具有统计学意义，其中三个影响应激指标和体重（在该群体的一个亚组中），一个推定的SNP影响生长性能，而未发现与抗病性相关的SNP。使用限制最大似然法（REML）估计了应激指标和体重的中度到高度基因组遗传力。最后，计算了所有性状的准确性以及估计育种值（EBV）和基因组估计育种值（GEBV）之间的相关性。
