Food Science Institute, Kansas State University, 1530 Mid-Campus Drive North, Manhattan, KS, 66506, USA.
Center for Biofilm Engineering, Montana State University, 366 Barnard Hall, Bozeman, MT, 59717, USA.
Food Microbiol. 2020 Dec;92:103592. doi: 10.1016/j.fm.2020.103592. Epub 2020 Jul 5.
Listeria monocytogenes is one of the most concerning pathogens for the food industry due to its ability to form biofilms, particularly in difficult-to-clean sites of processing facilities. There is a current industry-wide lack of data to refer to when selecting a strategy to control L. monocytogenes biofilms in the food premises. Many strategies have been developed to study biofilm formation of bacteria; however, few have targeted L. monocytogenes biofilms under dynamic conditions. This study addresses the biofilm formation ability of L. monocytogenes on stainless steel and polycarbonate under dynamic conditions using TSBYE or BHI as media culture at 30 °C or 37 °C. Higher cell counts were recovered at 30 °C in TSBYE on polycarbonate while lower counts were obtained at 37 °C in BHI on stainless steel (P < 0.05). Nonetheless, all factors (temperature, media and material) were statistically significant (P < 0.05) and an interaction between temperature and media was observed (P < 0.05). To our knowledge, this work represents an initial framework to develop L. monocytogenes biofilms under different dynamic conditions. The use of CDC Biofilm Reactor is not widely used yet in the food industry and represent a novel approach to help sanitary control strategies implementation.
李斯特菌是食品工业中最令人担忧的病原体之一，因为它能够形成生物膜，特别是在加工设施难以清洁的部位。目前，在选择控制食品场所李斯特菌生物膜的策略时，行业内缺乏参考数据。已经开发出许多策略来研究细菌的生物膜形成，但很少有针对动态条件下李斯特菌生物膜的策略。本研究使用 TSBYE 或 BHI 作为培养基，在 30°C 或 37°C 下，研究了李斯特菌在不锈钢和聚碳酸酯上的动态条件下的生物膜形成能力。在 TSBYE 中，聚碳酸酯上的细胞计数在 30°C 时较高，而在 BHI 中，不锈钢上的细胞计数在 37°C 时较低（P<0.05）。尽管如此，所有因素（温度、培养基和材料）均具有统计学意义（P<0.05），并且观察到温度和培养基之间存在相互作用（P<0.05）。据我们所知，这项工作代表了在不同动态条件下开发李斯特菌生物膜的初步框架。CDC 生物膜反应器在食品工业中尚未广泛使用，代表了一种帮助实施卫生控制策略的新方法。
