Beyazcicek Ersin, Ankarali Seyit, Beyazcicek Ozge, Ankarali Handan, Demir Serif, Ozmerdivenli Recep
Departments of Physiology, Medical School, Duzce University, Duzce, Turkey.
Neurosciences (Riyadh). 2016 Apr;21(2):131-7. doi: 10.17712/nsj.2016.2.20150781.
To investigate the effects of thymoquinone (TQ) in a penicillin-induced epilepsy model in rats.
This experimental study included 56 adult male Wistar rats. Experiments were performed in the Research Laboratory of the Department of Physiology, Medical School, Duzce University, Duzce, Turkey, between October 2013 and December 2014. Animals were divided into the following 7 groups: sham, control, only thymoquinone, vehicle (Dimethylsulfoxide), and doses of 10, 50, and 100 mg/kg of TQ. After rats were anesthetized, the left part of the skull was removed. A pair of silver/silver chloride electrodes was placed on the somatomotor area, and electrocorticographic recording was started. After 5 minutes basal activity was recorded, and TQ was applied intraperitoneally. At the thirtieth minute after TQ, epileptiform activity was induced by intracortical penicillin. The first spike latency, spike frequency, and the amplitude of epileptiform activity were analyzed statistically.
The different doses of TQ significantly increased the latency time to onset of first spike wave, and decreased the frequency, and amplitude of epileptiform activity in the first 20 minutes compared with the control group.
Thymoquinone shows potential as an antiepileptic drug resulting from its effects of prolonged latency time, and reduced spike wave frequency and amplitude of epileptiform activity.
研究百里醌（TQ）对青霉素诱导的大鼠癫痫模型的影响。
本实验研究纳入56只成年雄性Wistar大鼠。实验于2013年10月至2014年12月在土耳其杜兹大学医学院生理学系研究实验室进行。动物被分为以下7组：假手术组、对照组、仅给予百里醌组、溶剂（二甲基亚砜）组以及剂量为10、50和100mg/kg的百里醌组。大鼠麻醉后，去除颅骨左侧部分。将一对银/氯化银电极置于躯体运动区，开始进行皮质脑电图记录。记录5分钟基础活动后，腹腔注射百里醌。在注射百里醌后第30分钟，通过皮质内注射青霉素诱导癫痫样活动。对首次棘波潜伏期、棘波频率和癫痫样活动的幅度进行统计学分析。
与对照组相比，不同剂量的百里醌在前20分钟显著延长了首次棘波发作的潜伏期时间，并降低了癫痫样活动的频率和幅度。
百里醌具有作为抗癫痫药物的潜力，因其具有延长潜伏期时间、降低癫痫样活动的棘波频率和幅度的作用。
