Sericultural & Agri-Food Research Institute Guangdong Academy of Agricultural Sciences, Guangzhou, China; Key Laboratory of Urban Agriculture in South China, Ministry of Agriculture, Guangzhou, China.
Sericultural & Agri-Food Research Institute Guangdong Academy of Agricultural Sciences, Guangzhou, China.
Ecotoxicol Environ Saf. 2020 Dec 1;205:111298. doi: 10.1016/j.ecoenv.2020.111298. Epub 2020 Sep 17.
Mulberry (Morus atropurpurea) is an economically important woody tree and has great potential for the remediation of heavy metals. To investigate how cadmium accumulates and its detoxification in mulberry, we assessed the physiological and transcriptomic effects of cadmium contamination and as well as its chemical forms and subcellular distribution. Cadmium significantly inhibited mulberry plant growth and primarily accumulated in mulberry roots. Antioxidant enzymes were induced by cadmium in all tissues of mulberry. Subcellular fractionation analyses of cadmium indicated that the majority was compartmentalized in soluble fraction in roots while it mainly located in cell wall in leaves and stems. The greatest amount of the cadmium was integrated with proteins and pectates in all mulberry tissues. RNA-seq transcriptomic analyses of mulberry roots revealed that various metabolic pathways involved in cadmium stress response such as RNA regulation, hormone metabolism, and response to stress, secondary metabolism, as well as signaling, protein metabolism, transport, and cell-wall metabolism. These results will increase our understanding of the molecular mechanisms of cadmium detoxification in mulberry and provide new insights into engineering woody plants for phytoremediation.
桑树（Morus atropurpurea）是一种经济上重要的木本植物，对于重金属的修复具有巨大的潜力。为了研究镉在桑树上的积累及其解毒机制，我们评估了镉污染对桑树的生理和转录组的影响，以及其化学形态和亚细胞分布。镉显著抑制了桑树的生长，主要积累在桑树的根部。镉在桑树的所有组织中诱导了抗氧化酶。镉的亚细胞分离分析表明，大部分镉在根部的可溶性部分被分隔，而在叶片和茎部主要位于细胞壁中。在所有桑树组织中，最大量的镉与蛋白质和果胶结合。对桑树根部的 RNA-seq 转录组分析表明，各种与镉胁迫反应相关的代谢途径，如 RNA 调控、激素代谢和应激反应、次生代谢以及信号转导、蛋白质代谢、运输和细胞壁代谢，都参与其中。这些结果将增加我们对桑树中镉解毒的分子机制的理解，并为利用木本植物进行植物修复提供新的见解。
