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镉处理对青藏高原本地植物 Griseb. 形态、叶绿素含量和抗氧化酶活性的影响。

Effects of Cd treatment on morphology, chlorophyll content and antioxidant enzyme activity of Griseb., a native plant in Qinghai-Tibet Plateau.

机构信息

College of Life Sciences, Qinghai Normal University, Xi' Ning, China.

Qinghai south of Qilian Mountain Forest Ecosystem Observation and Research Station, Huzhu.

出版信息

Plant Signal Behav. 2023 Dec 31;18(1):2187561. doi: 10.1080/15592324.2023.2187561.

Abstract

Cd pollution is a global environmental problem. However, the response mechanism of the alpine plant Pelagia under Cd stress remains unclear. Therefore, in this study, a native plant( Griseb.) of the Qinghai-Tibet Plateau was used as the material to quantify plant height, leaf number, length of leaf, crown width, root number, biomass, Dry weight malondialdehyde (MDA), free proline, superoxide dismutase (SOD), ascorbate enzyme (APX), catalase (CAT) and chlorophyll contents under different Cd concentrations. The results showed that the growth of Griseb. was a phenomenon of "low concentration promotes growth, high concentration inhibited growth" under Cd treatment. It meant that 10 mg·L Cd promoted the growth of leaf number, plant height, crown width and tiller number, while 40 mg·L Cd inhibited the growth of root number and biomass of Griseb. compare with the control. The MDA content, free proline content, SOD activity, APX activity and CAT activity of Griseb. was increased with the increase of Cd treatment concentration to resist the oxidative damage caused by Cd to the body. At the same time, the accumulation of chlorophyll A, chlorophyll B and chlorophyll AB was decreased with the increase of Cd stress concentration. In addition, the carotenoid content did not change much between the control group and the treatment group, indicating that Cd treatment had little effect on it. The results could provide a reference for the mechanism of heavy metal resistance and the selection and improvement of Cd -resistant varieties of Griseb.

摘要

镉污染是一个全球性的环境问题。然而,高山植物在镉胁迫下的响应机制仍不清楚。因此,本研究以青藏高原的一种本地植物( Griseb.)为材料,定量研究了不同镉浓度下植物的株高、叶数、叶长、冠宽、根数、生物量、干重丙二醛(MDA)、游离脯氨酸、超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)、过氧化氢酶(CAT)和叶绿素含量。结果表明,在镉处理下, Griseb. 的生长表现出“低浓度促进生长,高浓度抑制生长”的现象。这意味着 10mg·L-1 Cd 促进了叶数、株高、冠宽和分蘖数的生长,而 40mg·L-1 Cd 抑制了根数和生物量的生长。与对照组相比。随着 Cd 处理浓度的增加, Griseb. 的 MDA 含量、游离脯氨酸含量、SOD 活性、APX 活性和 CAT 活性增加,以抵抗 Cd 对机体造成的氧化损伤。同时,随着 Cd 胁迫浓度的增加,叶绿素 A、叶绿素 B 和叶绿素 AB 的积累减少。此外,对照组和处理组之间类胡萝卜素含量变化不大,表明 Cd 处理对其影响不大。研究结果可为研究重金属抗性机制和 Griseb. 耐 Cd 品种的选择和改良提供参考。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/085c/10038041/db7f7d4e0df5/KPSB_A_2187561_F0001_OC.jpg

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