Ranjbar Reza, Safarpoor Dehkordi Farhad, Heiat Mohammad
Molecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Halal Research Center of IRI, FDA, Tehran, Iran.
Iran J Public Health. 2020 May;49(5):968-974.
causes infections in humans and animals. Antibiotics are used to eliminate bacterial infections, which become resistant to antibiotics after a while. This study aimed to isolate . from cattle feces samples and also to evaluate the frequency of genes associated with multi-drug resistance (MDR).
One hundred ten fecal samples of cattle were collected from Jul to Dec, 2017 in Khuzestan Province, southern Iran. Bacterial culture and molecular methods were used to isolate and identify . Disk diffusion method was used to assess antibiotic susceptibility. Then Polymerase chain reaction (PCR), assay was used for definitive diagnosis of . and resistance genes.
Overall, 101 (91.81%) samples were detected to be contaminated with genus and 86 samples (85.14%) were identified as . The highest and lowest antibiotic resistance were belonged to gentamicin (n=70, 81.39%), and tetracycline (n=6, 6.97%). Besides, 64 samples (74.42%) had 2-10 drugs resistance patterns. Moreover, the highest and the least resistance were related to (n=73, 84.88%) and (n=49, 56.97%) genes respectively.
The drug-induced genes in . have a high frequency. Therefore, antibiotic resistance and high MDR to antibiotics can be due to the incorrect use of antibiotics and the lack of health monitoring in Cattle farms.
可导致人类和动物感染。抗生素用于消除细菌感染，但细菌一段时间后会对抗生素产生耐药性。本研究旨在从牛粪样本中分离出……，并评估与多重耐药性（MDR）相关基因的频率。
2017年7月至12月，从伊朗南部胡齐斯坦省采集了110份牛粪样本。采用细菌培养和分子方法进行分离和鉴定……。采用纸片扩散法评估抗生素敏感性。然后使用聚合酶链反应（PCR）检测法对……和耐药基因进行确诊。
总体而言，101份（91.81%）样本被检测出受到……属污染，86份样本（85.14%）被鉴定为……。最高和最低的抗生素耐药率分别属于庆大霉素（n = 70，81.39%）和四环素（n = 6，6.97%）。此外，64份样本（74.42%）具有2 - 10种耐药模式。而且，最高和最低耐药率分别与……（n = 73，84.88%）和……（n = 49，56.97%）基因相关。
……中的耐药基因频率较高。因此，抗生素耐药性和对多种抗生素的高多重耐药性可能是由于抗生素使用不当以及养牛场缺乏健康监测所致。
