Department of Fisheries Microbiology, Karnataka Veterinary Animal and Fisheries Sciences University, College of Fisheries Mangalore, 575002, India.
Food Sci Nutr. 2014 Jul;2(4):436-42. doi: 10.1002/fsn3.119. Epub 2014 May 26.
Antimicrobial-resistant salmonellosis is a significant public health concern globally. A study was conducted to screen for Salmonella species from a total of 120 samples, of which 50 were retail meat samples purchased from five randomly selected sales outlets in the city of Mangalore, India. Twenty poultry fecal materials freshly voided before slaughter were obtained with sterile spatula and placed in sterile sealable polythene envelopes, and 20 clams were purchased from the estuaries of Nethravathi and Kankarnady market. In addition, 30 clinical isolates from Nigeria suspected to be Salmonella by only cultural characterization were also included in the study. In all, 30 samples-6 poultry, 8 seafood, and 16 Salmonella isolates from clinical samples-were confirmed positive by PCR and used in this study. The disk-diffusion test was performed to determine the zone of inhibition, and detection of resistance genes was tested by PCR targeting various antimicrobial genes. Resistance to tetracycline (TET), cotrimoxazole, nalidixic acid, nitrofurantion, and piperacillin/tazobactin was found in 66.7%, 60%, 53.3%, 50% and 50% of the isolates, respectively. About 60-100% of MDR isolates possessed antibiotic-resistant genes, of the tetracyclines resistant isolates, 20 (100%) 6 (30%), 7 (35%), and 10 (50%) carried tetA, tetB, tetC, and tetG genes, respectively. Of 18 cotrimoxazole-resistant strains, 18 (100%), 14 (77.7%), and 4 (22.2%) had sul1, sul2, and sul3 genes, respectively. Of the 14 multidrug-resistant isolates tested, 8 (61%) and 9 (69%) were positive for cmlA and cmlB genes, respectively, 10 (1.4%) tested positive for aph(3)11a genes, 8 (57%) tested positive for aac(3)lla, while none was positive for the aac6 gene. The results show the presence of antibiotic-resistant Salmonella spp. in food samples from India and in human samples from Nigeria.
抗药性沙门氏菌感染是全球公共卫生的重大关切。本研究旨在从总共 120 个样本中筛选沙门氏菌，其中 50 个样本是从印度芒格洛尔市五个随机选定的销售点购买的零售肉类样品。用无菌抹刀从 20 份家禽粪便中收集刚排出的新鲜粪便，并将其置于无菌密封的聚乙烯袋中，同时从内特拉瓦蒂和坎卡纳迪市场的河口购买 20 个蛤。此外，还包括来自尼日利亚的 30 个临床分离株，这些分离株仅通过文化特征被怀疑为沙门氏菌。总共有 30 个样本（6 个家禽、8 个海鲜和 16 个来自临床样本的沙门氏菌分离株）通过 PCR 确认为阳性，并用于本研究。通过纸片扩散试验确定抑菌圈大小，并通过针对各种抗菌基因的 PCR 检测来检测耐药基因。66.7%、60%、53.3%、50%和 50%的分离株分别对四环素（TET）、复方新诺明、萘啶酸、呋喃妥因和哌拉西林/他唑巴坦耐药。大约 60-100%的多药耐药分离株具有抗生素耐药基因，在四环素耐药的分离株中，20（100%）、6（30%）、7（35%）和 10（50%）分别携带 tetA、tetB、tetC 和 tetG 基因。在 18 株复方新诺明耐药株中，18（100%）、14（77.7%）和 4（22.2%）分别携带 sul1、sul2 和 sul3 基因。在 14 株多药耐药分离株中，8（61%）和 9（69%）对 cmlA 和 cmlB 基因呈阳性，10（1.4%）对 aph(3)11a 基因呈阳性，8（57%）对 aac(3)lla 呈阳性，而 aac6 基因均呈阴性。结果表明，在印度的食品样本和尼日利亚的人类样本中存在耐药性沙门氏菌。
