Tagge E P, Campbell D A, Reichle R, Averill D R, Merion R M, Dafoe D C, Turcotte J G, Juni J E
J Surg Res. 1987 Jun;42(6):605-12. doi: 10.1016/0022-4804(87)90003-5.
A mathematical technique known as deconvolutional analysis was used to provide a critical and previously missing element in the computations required to quantitate hepatic function scintigraphically. This computer-assisted technique allowed for the determination of the time required, in minutes, of a labeled bilirubin analog (99mTc-disofenin) to enter the liver via blood and exit via bile. This interval was referred to as the mean transit time (MTT). The critical process provided for by deconvolution is the mathematical simulation of a bolus injection of tracer directly into the afferent blood supply of the liver. The raw data required for this simulation are obtained from the intravenous injection of labeled disofenin, a member of the HIDA family of radiopharmaceuticals. In this study, we perform experiments which document that the simulation process itself is accurate. We then calculate the MTT under a variety of experimental conditions involving progressive hepatic ischemia/reperfusion injury and correlate these results with the results of simultaneously performed BSP determinations and hepatic histology. The experimental group with the most pronounced histologic findings (necrosis, vacuolization, disorganization of hepatic cords) also have the most prolonged MTT and BSP half-life. However, both quantitative imaging and BSP testing are able to identify milder degrees of hepatic ischemic injury not reflected in the histologic evaluation. Quantitative imaging with deconvolutional analysis is a technique easily adaptable to the standard nuclear medicine minicomputer. It provides rapid results and appears to be a sensitive monitor of hepatic functional disturbances resulting from ischemia and reperfusion.
一种称为去卷积分析的数学技术被用于提供在通过闪烁扫描法定量肝功能所需的计算中一个关键且此前缺失的要素。这种计算机辅助技术能够确定一种标记胆红素类似物（99mTc - 二异丙半胱氨酸）经血液进入肝脏并经胆汁排出所需的时间（以分钟计）。这个间隔被称为平均通过时间（MTT）。去卷积所提供的关键过程是对示踪剂直接注入肝脏传入血液供应的团注进行数学模拟。该模拟所需的原始数据是通过静脉注射标记的二异丙半胱氨酸（一种放射性药物HIDA家族的成员）获得的。在本研究中，我们进行了实验，证明模拟过程本身是准确的。然后我们在各种涉及进行性肝缺血/再灌注损伤的实验条件下计算MTT，并将这些结果与同时进行的BSP测定结果和肝脏组织学结果相关联。组织学发现最明显（坏死、空泡化、肝索紊乱）的实验组也具有最长的MTT和BSP半衰期。然而，定量成像和BSP检测都能够识别出组织学评估中未反映的较轻程度的肝缺血损伤。采用去卷积分析的定量成像是一种易于适配标准核医学小型计算机的技术。它能快速得出结果，并且似乎是缺血和再灌注导致的肝功能紊乱的敏感监测手段。
